Project/scholarship details
- Project/scholarship ID
102608
- Reference
PTDC/AGR-ALI/102608/2008
- FundRef URI
- Funder
FCT - Fundação para a Ciência e a Tecnologia, I.P.
- Funder's country
Portugal
- Funding program
3599-PPCDT
- Funding amount
193,648.00 €
- Start date
2010-04-01
- End date
2013-09-30
Documents
Identification of candidate genes for yeast engineering to improve bioethanol p...
Pereira, Francisco B.; Guimarães, Pedro M. R.; Gomes, Daniel Gonçalves; Mira, Nuno P.; Teixeira, Miguel C.; Sá-Correia, Isabel; Domingues, LucíliaBackground: The optimization of industrial bioethanol production will depend on the rational design and manipulation of industrial strains to improve their robustness against the many stress factors affecting their performance during very high gravity (VHG) or lignocellulosic fermentations. In this study, a set of Saccharomyces cerevisiae genes found, through genome-wide screenings, to confer resistance to the ...
Ammonium blocks chronological lifespan extension of extreme calorie restriction...
Santos, Júlia; Sousa, Maria João; Leão, CecíliaWe have previously shown that ammonium stimulates cell death in amino acid-deprived auxotrophic cells of Saccharomyces cerevisiae (aa-starved cells) transferred to water, whereas the same cells in absence of ammonium displayed chronological lifespan (CLS) expansion typical of extreme starvation conditions. The enhanced loss of cell viability in the presence of ammonium was accompanied by an initial small increa...
Protective effect of acetic acid against ethanol-induced cell death in "Sacchar...
Afonso, Andreia FernandesDissertação de mestrado em Genética Molecular; O etanol é um produto final bem conhecido da fermentação alcoólica realizada por Saccharomyces cerevisiae. Em altas concentrações, é responsável pela redução de viabilidade celular e inibição da fermentação. Além disso, durante a fermentação alguns ácidos fracos, como os ácidos acético, butírico e pirúvico, produzidos pelo metabolismo da levedura, podem acumular-se...
Ammonium Is toxic for aging yeast cells, inducing death and shortening of the c...
Santos, Júlia; Sousa, Maria João; Leão, CecíliaHere we show that in aging Saccharomyces cerevisiae (budding yeast) cells, NH4+ induces cell death associated with shortening of chronological life span. This effect is positively correlated with the concentration of NH4+ added to the culture medium and is particularly evident when cells are starved for auxotrophy-complementing amino acids. NH4+-induced cell death is accompanied by an initial small increase of ...
The fate of acetic acid during glucose co-metabolism by the spoilage yeast Zygo...
Rodrigues, Fernando José dos Santos; Sousa, Maria João; Ludovico, Paula; Santos, Helena; Côrte-Real, Manuela; Leão, CecíliaZygosaccharomyces bailii is one of the most widely represented spoilage yeast species, being able to metabolise acetic acid in the presence of glucose. To clarify whether simultaneous utilisation of the two substrates affects growth efficiency, we examined growth in single- and mixed-substrate cultures with glucose and acetic acid. Our findings indicate that the biomass yield in the first phase of growth is the...
Growth culture conditions and nutrient signaling modulating yeast chronological...
Santos, Júlia; Leão, Cecília; Sousa, Maria JoãoThe manipulation of nutrient-signaling pathways in yeast has uncovered the impact of environmental growth conditions in longevity. Studies using calorie restriction show that reducing glucose concentration of the culture media is sufficient to increase replicative and chronological lifespan (CLS). Other components of the culture media and factors such as the products of fermentation have also been implicated in...
Identification of genes and the signal transduction pathways involved in the re...
Duarte, Ana Marta GomesDissertação de mestrado em Genética Molecular; After many years of research, S. cerevisiae was accepted as a powerful model that allows increasing our comprehension about the underlying mechanisms of apoptosis in more complex and less accessible organisms. So, to better understand these apoptotic mechanisms we performed a functional analysis, at whole-genome scale, with the Euroscarf mutants collection. This an...
Genome-wide identification of genes involved in the positive and negative regul...
Sousa, Marlene; Duarte, Ana Marta Gomes; Fernandes, Tânia Alícia Ribeiro; Chaves, S. R.; Pacheco, Andreia; Leão, Cecília; Côrte-Real, ManuelaBackground: Acetic acid is mostly known as a toxic by-product of alcoholic fermentation carried out by Saccharomyces cerevisiae, which it frequently impairs. The more recent finding that acetic acid triggers apoptotic programmed cell death (PCD) in yeast sparked an interest to develop strategies to modulate this process, to improve several biotechnological applications, but also for biomedical research. Indeed,...
Ammonium-dependent shortening of CLS in yeast cells starved for essential amino...
Santos, Júlia; Leão, Cecília; Sousa, Maria JoãoAmmonium (NH4(+)) leads to chronological life span (CLS) shortening in Saccharomyces cerevisiae BY4742 cells, particularly evident in cells starved for auxotrophy-complementing amino acids (leucine, lysine, and histidine) simultaneously. Here, we report that the effect of NH4(+) on aging yeast depends on the specific amino acid they are deprived of. Compared with no amino acid starvation, starvation for leucine...
The genome sequence of the highly acetic acid-tolerant zygosaccharomyces bailii...
Leão, Cecília; Rodrigues, Fernando José dos Santos; Santos, Júlia; Mira, Nuno P.; Münsterkötter, Martin; Valada, Filipa Dias; Palma, MargaridaIn this work, it is described the sequencing and annotation of the genome of the yeast strain ISA1307, isolated from a sparkling wine continuous production plant. This strain, formerly considered of the Zygosaccharomyces bailii species, has been used to study Z. bailii physiology, in particular, its extreme tolerance to acetic acid stress at low pH. The analysis of the genome sequence described in this work ind...
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