Project/scholarship details
- Project/scholarship ID
125757
- Reference
FCT-ANR/BEX-BCM/0175/2012
- FundRef URI
- Funder
FCT - Fundação para a Ciência e a Tecnologia, I.P.
- Funder's country
Portugal
- Funding program
3599-PPCDT
- Funding amount
185,036.00 €
- Start date
2013-04-01
- End date
2016-09-30
Documents
Contribution of the adenine nucleotide carrier, porin, and sphingolipid metabol...
Trindade, Dário Alexandre MartinsTese de doutoramento do Programa Doutoral em Biologia Molecular e Ambiental (especialidade em Biologia Celular e Saúde); A decisive event in the cell‟s life-or-death decision is the mitochondrial outer membrane permeabilization (MOMP). The biochemical events responsible for MOMP, considered by many investigators the “point-of-no-return” in cell death, are not entirely defined. Two major and very distinct mechan...
Impact of mutated KRAS signalling on autophagy regulation in colorectal carcinoma
Alves, Sara Cristina SequeiraPrograma Doutoral em Biologia Molecular e Ambiental; Colorectal carcinoma (CRC) is the third most commonly diagnosed cancer worldwide and one of the most abundant causes of cancer related deaths. KRAS mutations ( KRASG13D, KRASG12D and KRASG12V) are frequent events in CRC, detected in about 30% to 50% of the clinical cases, and seem to play an important role in colon carcinogenesis. It is known that KRAS mutati...
Colorectal cancer-related mutant KRAS alleles function as positive regulators o...
Alves, Sara; Castro, Lisandra; Fernandes, Maria Sofia; Francisco, Rita; Castro, Paula; Priault, Muriel; Chaves, Susana Rodrigues; Moyer, Mary PatThe recent interest to modulate autophagy in cancer therapy has been hampered by the dual roles of this conserved catabolic process in cancer, highlighting the need for tailored approaches. Since RAS isoforms have been implicated in autophagy regulation and mutation of the KRAS oncogene is highly frequent in colorectal cancer (CRC), we questioned whether/how mutant KRAS alleles regulate autophagy in CRC and its...
Yeast as a tool to explore cathepsin D function
Pereira, H; Oliveira, C S F; Castro, L.; Preto, Ana; Chaves, S. R.; Côrte-Real, ManuelaCathepsin D has garnered increased attention in recent years, mainly since it has been associated with several human pathologies. In particular, cathepsin D is often overexpressed and hypersecreted in cancer cells, implying it may constitute a therapeutic target. However, cathepsin D can have both anti- and pro-survival functions depending on its proteolytic activity, cellular context and stress stimulus. There...
Modulation of Bax by PKCε: an approach to eliminate cancer cells
Pereira, Soraia Raquel AzevedoDissertação de mestrado em Bioquímica Aplicada; Apoptosis is a type of cell death, known as a highly regulated program of cellular suicide; however this process is often deregulated in tumor cells. A mechanism used by tumor cells to evade apoptosis is the alteration of the different members of the Bcl-2 family. These proteins control a key step in the intrinsic apoptotic pathway: permeabilization of the outer m...
Characterization of acetate transport in colorectal cancer cells and potential ...
Ferro, Suellen; Azevedo-Silva, João; Casal, Margarida; Côrte-Real, Manuela; Baltazar, Fátima; Preto, AnaAcetate, together with other short chain fatty acids has been implicated in colorectal cancer (CRC) prevention/therapy. Acetate was shown to induce apoptosis in CRC cells. The precise mechanism underlying acetate transport across CRC cells membrane, that may be implicated in its selectivity towards CRC cells, is not fully understood and was addressed here. We also assessed the effect of acetate in CRC glycolyti...
Lactoferrin selectively triggers apoptosis in highly metastatic breast cancer c...
Pereira, Cátia S.; Guedes, J.; Gonçalves, Marília; Loureiro, Luís; Castro, Lisandra; Gerós, Hernâni; Rodrigues, L. R.; Côrte-Real, ManuelaBreast cancer is the most common type of cancer affecting women. Despite the good prognosis when detected early, significant challenges remain in the treatment of metastatic breast cancer. The recruitment of the vacuolar H+-ATPase (V-H+-ATPase) to the plasma membrane, where it mediates the acidification of the tumor microenvironment (TME), is a recognized feature involved in the acquisition of a metastatic phen...
Proteasome inhibition prevents cell death induced by the chemotherapeutic agent...
Costa, Ana Rita Martins; Machado, Nuno; Rego, António; Sousa, Maria João; Côrte-Real, Manuela; Chaves, Susana Alexandra RodriguesCisplatin is a highly effective chemotherapeutic drug acting as a DNA-damaging agent that induces apoptosis of rapidly proliferating cells. Unfortunately, cellular resistance still occurs. Mutations in p53 in a large fraction of tumor cells contribute to defects in apoptotic pathways and drug resistance. To uncover new strategies to eliminate tumors through a p53-independent pathway, we established a simplified...
Acetic acid triggers cytochrome c release in yeast heterologously expressing hu...
Guedes, Joana P.; Baptista, Vitória; Cátia S. Pereira; Sousa, Maria João; Manon, Stéphen; Chaves, Susana R.; Côrte-Real, ManuelaProteins of the Bcl-2 protein family, including pro-apoptotic Bax and anti-apoptotic Bcl-xL, are critical for mitochondrial-mediated apoptosis regulation. Since yeast lacks obvious orthologs of Bcl-2 family members, heterologous expression of these proteins has been used to investigate their molecular and functional aspects. Active Bax is involved in the formation of mitochondrial outer membrane pores, through ...
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