Project/scholarship details
- Project/scholarship ID
103392
- Reference
PTDC/AGR-ALI/103392/2008
- FundRef URI
- Funder
FCT - Fundação para a Ciência e a Tecnologia, I.P.
- Funder's country
Portugal
- Funding program
5876-PPCDTI
- Funding amount
156,676.00 €
- Start date
2010-06-01
- End date
2013-11-30
Documents
Effects of acetic acid, ethanol and SO2 on the removal of volatile acidity from...
Moura, A. Vilela; Schuller, Dorit Elisabeth; Faia, A. Mendes; Côrte-Real, ManuelaHerein we report the influence of different combinations of initial concentration of acetic acid and ethanol on the removal of acetic acid from acidic wines by two commercial Saccharomyces cerevisiae strains S26 and S29. Both strains reduced the volatile acidity of an acidic wine (1.0 g l-1 acetic acid and 11% (v/v) ethanol) by 78% and 48%, respectively. Acetic acid removal by both strains was associated with a...
A colecção de estirpes autóctones de Saccharomyces cerevisiae das principais re...
Vieira, E.; Neves, J. Drumonde; Machado, Raul; Silva, Paulo; Gomes, Ana Catarina; Sousa, Susana; Ramos, P.; Alemão, F.; Lima, Maria TeresaLivro de Actas on-line em http://www.ateva.pt/8-simposio-de-vitivinicultura-do-alentejo/; A levedura Saccharomyces cerevisiae é caracterizada por uma elevada variabilidade fenotípica, que está associada à ocorrência de estirpes em habitats naturais diversificados. Em trabalhos anteriores, demonstrámos a elevada diversidade de estirpes isoladas a partir de ambientes vitivinícolas na Região dos Vinhos Verdes, bem...
Effect of refermentation conditions and microoxygenation on the reduction of vo...
Moura, A. Vilela; Schuller, Dorit Elisabeth; Faia, A. Mendes; Côrte-Real, Manuela; Falco, V.Herein, we evaluate the applicability of previously characterized commercial and indigenous Saccharomyces cerevisiae strains and non-S. cerevisiae species for the deacidification of white and red wines at a pilot scale. The effect of the refermentation process (mixture of acidic wine with musts from freshly crushed grapes or with residual marc) as well as MO (MO) on acetic acid removal efficiency and wine aroma...
Genotyping of Saccharomyces cerevisiae strains by interdelta sequence typing us...
Duarte, Ricardo Franco; Gomes, Ana Catarina; Santos, Manuel A. S.; Sousa, Bruno; Schuller, Dorit Elisabeth; Mendes, InêsAmplification of genomic sequences flanked by delta elements of retrotransposons TY1 and TY2 is a reliable method for characterization of Saccharomyces cerevisiae strains. The aim of this study is to evaluate the usefulness of microfluidic electrophoresis (Caliper LabChip®) to assess the factors that affect interlaboratory reproducibility of interdelta sequence typing for S. cerevisiae strain delimitation. We c...
A new high-throughput method for the rapid extraction of yeast DNA
Vieira, E.; Neves, J. Drumonde; Lima, Maria Teresa; Araújo, Isabel M.; Casal, Margarida; Schuller, Dorit ElisabethEcological, evolutionary and population genetic studies of yeasts require the processing of a high numbers of isolates. The current molecular methods for yeast identification and characterization require time-consuming and labor intensive DNA extraction protocols. Within this research, we aimed to develop a high-throughput method for yeast DNA extraction. Total yeast DNA extraction was based on a previously des...
Genetic diversity and population structure of Saccharomyces cerevisiae strains ...
Schuller, Dorit Elisabeth; Cardoso, Filipa; Sousa, Susana; Gomes, Paula; Gomes, Ana Catarina; Santos, Manuel A. S.; Casal, MargaridaWe herein evaluate intraspecific genetic diversity of fermentative vineyard-associated S. cerevisiae strains and evaluate relationships between grape varieties and geographical location on populational structures. From the musts obtained from 288 grape samples, collected from two wine regions (16 vineyards, nine grape varieties), 94 spontaneous fermentations were concluded and 2820 yeast isolates were obtained ...
Yeast biodiversity in vineyard environments is increased by human intervention
Drumonde-Neves, Joao; Franco-Duarte, Ricardo; Lima, Teresa; Schuller, Dorit; Pais, CéliaOne hundred and five grape samples were collected during two consecutive years from 33 locations on seven oceanic islands of the Azores Archipelago. Grape samples were obtained from vineyards that were either abandoned or under regular cultivation involving common viticultural interventions, to evaluate the impact of regular human intervention on grape yeast biota diversity in vineyards. A total of 3150 yeast i...
Association between Grape Yeast Communities and the Vineyard Ecosystems
Drumonde-Neves, Joao; Franco-Duarte, Ricardo; Lima, Teresa; Schuller, Dorit; Pais, CéliaThe grape yeast biota from several wine-producing areas, with distinct soil types and grapevine training systems, was assessed on five islands of Azores Archipelago, and differences in yeast communities composition associated with the geographic origin of the grapes were explored. Fifty-seven grape samples belonging to the Vitis vinifera grapevine cultivars Verdelho dos Acores (Verdelho), Arinto da Terceira (Ar...
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