Project/scholarship details
- Project/scholarship ID
110812
- Reference
MIT/ECE/0047/2009
- FundRef URI
- Funder
FCT - Fundação para a Ciência e a Tecnologia, I.P.
- Funder's country
Portugal
- Funding program
5876-PPCDTI
- Funding amount
199,800.00 €
- Start date
2010-06-01
- End date
2013-11-30
Documents
Micro/nano-scale strategies for engineering in vitro the celular microenvironme...
Coutinho, Daniela F.Programa doutoral em Bioengenharia; Biological tissues result of a specific spatial organization of cells, extracellular matrix (ECM) molecules, and soluble factors. These micro and nanoscaled biological entities organize into regional tissue architectures, creating highly complex and heterogeneous cellular microenvironments. To generate functional tissue equivalents in vitro, engineered biomaterials should mim...
Photocrosslinkable Kappa-carrageenan hydrogels for tissue engineering applications
Mihaila, Silvia M.; Gaharwar, Akhilesh K.; Reis, R. L.; Marques, A. P.; Gomes, Manuela E.; Khademhosseini, AliPublished online; Kappa carrageenan ( κ -CA) is a natural-origin polymer that closely mimics the glycosaminoglycan structure, one of the most important constituents of native tissues extracellular matrix. Previously, it has been shown that κ -CA can crosslink via ionic interactions rendering strong, but brittle hydrogels. In this study, we introduce photocrosslinkable methacrylate moieties on the κ -CA backbone...
Engineering articular cartilage using newly developed carrageenan basedhydrogels
Popa, Elena GetaTese doutoramento Programa Doutoral em Engenharia de Tecidos, Medicina Regenerativa e Células Estaminais; Articular cartilage holds specific functionality in the human body creating smooth gliding areas and allowing the joints to move easily without pain. However, due to its avascular nature and to the low metabolic activity of the constituent cells-the chondrocytes, cartilage has a low regenerative potential. ...
Fabrication of endothelial cell-laden carrageenan microfibers for microvascular...
Mihaila, Silvia M.; Popa, Elena Geta; Reis, R. L.; Marques, A. P.; Gomes, M. E.ecent achievements in the area of tissue engineering (TE) have enabled the development of three-dimensional (3D) cell-laden hydrogels as in vitro platforms that closely mimic the 3D scenario found in native tissues. These platforms are extensively used to evaluate cellular behavior, cell-cell interactions, and tissue-like formation in highly defined settings. In this study, we propose a scalable and flexible 3D...
Undifferentiated human adipose-derived stromal/stem cells loaded onto wet-spun ...
Carvalho, P. P.; Leonor, I. B.; Smith, Brenda J.; Dias, Isabel R.; Reis, R. L.; Gimble, Jeffrey M.; Gomes, Manuela E.The repair of large bony defects remains challenging in the clinical setting. Human adipose-derived stromal/ stem cells (hASCs) have been reported to differentiate along different cell lineages, including the osteogenic. The objective of the present study was to assess the bone regeneration potential of undifferentiated hASCs loaded in starchpolycaprolactone (SPCL) scaffolds, in a critical-sized nude mice calva...
The osteogenic differentiation of SSEA-4 sub-population of human adipose derive...
Mihaila, Silvia M.; Gaharwar, Akhilesh K.; Reis, R. L.; Khademhosseini, Ali; Marques, A. P.; Gomes, Manuela E.How to surpass invitro stem cell differentiation, reducing cell manipulation, and lead the in situ regeneration process after transplantation, remains to be unraveled in bone tissue engineering (bTE). Recently, we showed that the combination of human bone marrow stromal cells with bioactive silicate nanoplatelets (sNPs) promotes the osteogenic differentiation without the use of standard osteogenic inductors. Ev...
Seaweed polysaccharide-based hydrogels used for the regeneration of articular c...
Popa, Elena Geta; Reis, R. L.; Gomes, Manuela E.This manuscript provides an overview of the in vitro and in vivo studies reported in the literature focusing on seaweed polysaccharides based hydrogels that have been proposed for applications in regenerative medicine, particularly, in the field of cartilage tissue engineering. For a better understanding of the main requisites for these specific applications, the main aspects of the native cartilage structure, ...
Magnetically-responsive hydrogels for modulation of chondrogenic differentiation
Popa, Elena Geta; Santo, Vitor E.; Rodrigues, M. T.; Gomes, Manuela E.Magnetic nanoparticles (MNPs) are attractive tools to overcome limitations of current regenerative medicine strategies, demonstrating potential to integrate therapeutic and diagnostic functionalities in highly controlled systems. In traditional tissue engineering (TE) approaches, the MNPs association with stem cells in a three-dimensional (3D) template offers the possibility to achieve a mechano-magnetic respon...
Interactive endothelial phenotype maintenance and osteogenic differentiation of...
Mihaila, Silvia M.; Resende, Margarida F.; Reis, R. L.; Gomes, Manuela E.; Marques, A. P."Version of Record online: 29 OCT 2015"; Bone formation relies on complex processes that require well-orchestrated interactions between several cell types, such as bone-forming cells (osteoblasts, OBs) and endothelial cells (ECs). Their co-culture has been proved relevant to mimicking specific features of the bone niche. Here we propose the co-culture of microvascular-like ECs and pre-OBs, both derived from the...
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