Project/scholarship details
- Project/scholarship ID
113196
- Reference
PTDC/SAU-SAP/113196/2009
- FundRef URI
- Funder
FCT - Fundação para a Ciência e a Tecnologia, I.P.
- Funder's country
Portugal
- Funding program
5876-PPCDTI
- Funding amount
125,421.00 €
- Start date
2011-03-01
- End date
2014-08-31
Documents
New prophylactic strategies against Pseudomonas aeruginosa biofilms based on an...
Jorge, Paula Alexandra Silva; Lourenço, Anália Maria Garcia; Pereira, Maria Olívia[Excerpt] Introduction The solution to clinically relevant biofilms and antimicrobial resistance may lie in alternative products with new mechanisms of action. Antimicrobial peptides (AMPs) may be good replacements for current antibiotics because their action is low in specificity, reducing the chance of acquiring resistance, and they have a broad spectrum of activity against Gram-negative and -positive bacteri...
Preparation of a standardized methodology for the growth of Pseudomonas aerugin...
Jorge, Paula Alexandra SilvaDissertação de mestrado em Bioengenharia; Novel antimicrobial products, such as antimicrobial peptides (AMPs), are being investigated to combat clinically relevant biofilms and their growing antimicrobial resistance. These relevant studies, however, lack protocol standardization. Acknowledging the importance of standardized methodologies to ensure the quality of experimental results, this dissertation initiates...
Facilitating the screening of AMP literature via text mining strategies
Jorge, Paula Alexandra Silva; Pereira, Maria Olívia; Lourenço, Anália Maria Garcia[Excerpt] Introduction Antimicrobial peptides (AMPs) are nowadays importantly recognized, mostly due to the raise of infections caused by multi-resistant pathogens, and have been hailed as a potential solution to the shortage of novel antibiotics, due to their general unspecific mechanism of action which impairs the acquiring of resistance.; The financial support from IBB-CEB, Fundação para a Ciência e Tecnolog...
An harmonised vocabulary for communicating and interchanging Biofilms experimen...
Sousa, Ana Margarida; Pereira, Maria Olívia; Azevedo, N. F.; Lourenço, AnáliaBiofilm studies are at the crossroads of Biology, Chemistry, Medicine, Material Science and Engineering, among other fields. Data harmonisation in Biofilms is therefore crucial to allow for researchers to collaborate, interchange, understand, and replicate studies at an inter-laboratory and inter-domain scale. The international Minimum Information About a Biofilms Experiment initiative has prepared a set of gui...
Data quality in biofilm high-throughput routine analysis: intralaboratory proto...
Jorge, Paula Alexandra Silva; Lourenço, Anália; Pereira, Maria OlíviaBiofilm research is growing more diverse and dependent on high-throughput technologies and the large-scale production of results aggravates data substantiation. In particular, it is often the case that experimental protocols are adapted to meet the needs of a particular laboratory and no statistical validation of the modified method is provided. This paper discusses the impact of intra-laboratory adaptation and...
Polydopamine-mediated immobilization of alginate lyase to prevent P. aeruginosa...
Alves, D.; Sileika, Tadas; Messersmith, Phillip; Pereira, Maria OlíviaGiven alginate’s contribution to Pseudomonas aeruginosa virulence, it has long been considered a promising target for interventional therapies, which have been performed by using the enzyme alginate lyase. In this work, instead of treating pre-established mucoid biofi lms, alginate lyase is immobilized onto a surface as a preventive measure against P. aeruginosa adhesion. A polydopamine dip-coating strategy is e...
Effectiveness of colistin-antimicrobial peptides combinations against Pseudomon...
Jorge, Paula Alexandra Silva; Grzywacz, D.; Kamysz, W.; Lourenço, Anália Maria Garcia; Pereira, Maria OlíviaAntimicrobial research is increasingly being focused on the problem of microbial resistance and biofilm formation. Alternative mechanisms of action, as those of antimicrobial peptides (AMP), and synergic antimicrobial combinations can lead to more effective therapeutic strategies for this ever-growing world-wide problem. Hence, this work aimed to evaluate the antimicrobial and cytotoxic potential of combination...
Reconstruction of the network of experimentally validated AMP-drug combinations...
Jorge, Paula; Pérez-Pérez, Martín; Rodríguez, Gael Pérez; Fdez-Riverola, Florentino; Pereira, Maria Olívia; Lourenço, AnáliaThe combination of antimicrobial products is a promising biomedical strategy against the ever growing number of resistant strains emerging in healthcare and community settings. Agents with alternative modes of action, such as antimicrobial peptides (AMPs), and the efficacy of combined actions are being evaluated. Despite the availability of various antimicrobial data repositories, a wealth of information remain...
Insights into Pseudomonas aeruginosa quorum sensing therapeutics through text- ...
Jorge, Paula Alexandra Silva; Pérez-Pérez, Martín; Rodríguez, Gael Pérez; Pereira, Maria Olívia; Lourenço, Anália Maria GarciaThe unceasing emergence of antibiotic-resistant strains and resilient biofilm-related infections is pressing researchers to develop novel antimicrobial strategies. Quorum sensing (QS) is a key communication mechanism that holds promise as target to control clinical pathogens. QS allows bacteria to regulate gene expression, and thus many physiological activities, such as virulence, motility, and biofilm formatio...
Bio-inspired coating strategies for the immobilization of polymyxins to generat...
Alves, Diana Filipa Barros; Pereira, Maria OlíviaMicrobial colonization of indwelling devices remains a major concern in modern healthcare. Developing approaches to prevent biomaterial-associated infections (BAI) is, therefore, in great demand. This study aimed to immobilize two antimicrobial peptides (polymyxins B and E) onto polydimethylsiloxane (PDMS) using two polydopamine (pDA)-based approaches: the conventional two-step method involving the deposition o...
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