Project/scholarship details
- Project/scholarship ID
115179
- Reference
PTDC/SAU-ENB/115179/2009
- FundRef URI
- Funder
FCT - Fundação para a Ciência e a Tecnologia, I.P.
- Funder's country
Portugal
- Funding program
5876-PPCDTI
- Funding amount
149,456.00 €
- Start date
2011-01-01
- End date
2014-06-30
Documents
Liposomes in tissue engineering and regenerative medicine
Monteiro, Nelson; Martins, Albino; Reis, R. L.; Neves, N. M.Liposomes are vesicular structures made of lipids that are formed in aqueous solutions. Structurally, they resemble the lipid membrane of living cells. Therefore, they have been widely investigated, since the 1960s, as models to study the cell membrane, and as carriers for protection and/or delivery of bioactive agents. They have been used in different areas of research including vaccines, imaging, applications...
Highly functional and biodegradable nanofibrous scaffolds combined with stem ce...
Neves, N. M.Among the various possible embodiements of Advanced Therapies and in particular of Tissue Engineering the use of temporary scaffolds to regenerate tissue defects is one of the key issues. The scaffolds should be specifically designed to create environments that promote tissue development and not merely to support the maintenance of communities of cells. To achieve that goal, highly functional scaffolds may comb...
In vitro degradation and in vivo biocompatibility of chitosan-poly(butylene suc...
Pinto, A. R.; Martins, Ana M.; Carlos, Magda João Castelhano; Correlo, V. M.; Sol, P.; Longatto Filho, Adhemar; Battacharya, Mrinal; Reis, R. L.In tissue engineering, the evaluation of the host response to the biomaterial implantation must be assessed to determine the extent of the inflammatory reaction. We studied the degradation of poly(butylene succinate) and chitosan in vitro using lipase and lysozyme enzymes, respectively. The subcutaneous implantation of the scaffolds was performed to assess tissue response. The type of inflammatory cells present...
Influence of scaffold composition over in vitro osteogenic differentiation of h...
Pinto, A. R.; Vargel, I.; Tuzlakoglu, K.; Correlo, V. M.; Sol, P. C.; Faria, Susana; Piskin, Erhan; Reis, R. L.; Neves, N. M.To understand the role of chitosan in chitosan-poly(butylene succinate) scaffolds (50% wt), 50%, 25%, and 0% of chitosan were used to produce different scaffolds. These scaffolds were in vitro seeded and cultured with human bone marrow stromal cells in osteogenic conditions, revealing that higher percentage of chitosan showed enhanced cell viability over time, adhesion, proliferation, and osteogenic differentia...
Instructive nanofibrous scaffold comprising runt-related transcription factor 2...
Monteiro, Nelson; Ribeiro, Diana Margarida da Costa; Martins, Albino; Faria, Susana; Fonseca, Nuno A.; Moreira, João N.; Reis, R. L.; Neves, N. M."Epub 2014 Jul. 30"; Inducer molecules capable of regulating mesenchymal stem cells (MSCs) differentiation into specific lineages have proven effective in basic science and in preclinical studies. Runt-related transcription factor 2 (RUNX2) is considered to be the central gene involved in the osteoblast phenotype induction, which may be advantageous for inducing bone tissue regeneration. This work envisions the...
Nanoparticle-based bioactive agent release systems for bone and cartilage tissu...
Monteiro, Nelson; Martins, Albino; Reis, R. L.; Neves, N. M.The inability to deliver bioactive agents locally in a transient but sustained manner is one of the challenges on the development of bio-functionalized scaffolds for tissue engineering (TE) and regenerative medicine. The mode of release is especially relevant when the bioactive agent is a growth factor (GF), because the dose and the spatiotemporal release of such agents at the site of injury are crucial to achi...
Antibacterial activity of chitosan nanofiber meshes with liposomes immobilized ...
Monteiro, Nelson; Martins, Margarida; Martins, Albino; Fonseca, Nuno A.; Moreira, João N.; Reis, R. L.; Neves, N. M.Chitsan (Ch) nanofiber mesh (NFM) is a material with natural characteristics favoring its use in human wound dressing. The present work proposes a gentamicin-loaded liposome immobilized at the surface of Ch NFMs to promote its antibacterial activity. To achieve this purpose, Ch NFMs were functionalized with thiol groups, and gentamicin-loaded liposomes were covalently immobilized by the reaction of the SH group...
Dual release of a hydrophilic and a hydrophobic osteogenic factor from a single...
Monteiro, N.; Martins, A.; Pires, R. A.; Faria, S.; Fonseca, N. A.; Moreira, J. N.; Reis, R. L.; Neves, N. M.Delivery systems may be designed to protect and control the release kinetics of growth/differentiation factors in a spatiotemporal manner. Liposomes are examples of biological-based bioactive agent delivery systems. In this work, ascorbic acid (AscA) was encapsulated in the inner compartment of the liposome and dexamethasone (Dex) was encapsulated within the lipid bilayer in order to develop a dual release syst...
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