Project/scholarship details
- Project/scholarship ID
115977
- Reference
PTDC/CTM-BPC/115977/2009
- FundRef URI
- Funder
FCT - Fundação para a Ciência e a Tecnologia, I.P.
- Funder's country
Portugal
- Funding program
5876-PPCDTI
- Funding amount
136,125.00 €
- Start date
2011-01-01
- End date
2013-12-31
Documents
De novo bone formation on macro/microporous silk and silk/nano-sized calcium ph...
Yan, Leping; Salgado, A. J.; Oliveira, Joaquim M.; Oliveira, A. L.; Reis, R. L.Macro/micro porous silk/nano-sized calcium phosphate scaffolds (SC16) with bioactive and superior physicochemical properties have been recently developed. In this study, we aim at evaluating the new bone formation ability of the SC 16 scaffolds in vivo, using silk fibroin scaffolds (S16) as control. The CaP distribution profile in the scaffolds was characterized by Micro-Computed Tomography. The in vitro minera...
Current concepts: tissue engineering and regenerative medicine applications in ...
Correia, Sandra I.; Pereira, H.; Silva-Correia, Joana; Dijk, C. N. Van; Mendes, João Espregueira; Oliveira, Joaquim M.; Reis, R. L.Tissue engineering and regenerative medicine (TERM) has caused a revolution in present and future trends of medicine and surgery. In different tissues, advanced TERM approaches bring new therapeutic possibilities in either general population as in young patients and high-level athletes, improving restoration of biological functions and rehabilitation. The mainstream components required to obtain a functional re...
Gellan gum-based hydrogel bilayered scaffolds for osteochondral tissue engineering
Pereira, D. R.; Canadas, Raphael Faustino; Silva-Correia, Joana; Marques, A. P.; Reis, R. L.; Oliveira, Joaquim M.It has been shown that hydrogel bilayered scaffolds combining cartilage- and bone-like layers are most advantageous for treating osteochondral defects. In this study, it is proposed the use of low acyl gellan gum (LAGG) for developing bilayered hydrogel scaffolds for osteochondral tissue engineering. The cartilage-like layer of the GG-based bilayered hydrogel scaffolds is composed of LAGG (2 wt%). By adding a 2...
Silk Fibroin/Nano-CaP Bilayered scaffolds for osteochondral tissue engineering
Yan, Leping; Oliveira, Joaquim M.; Oliveira, A. L.; Reis, R. L.In this study, bilayered silk and silk/nano-CaP scaffolds were developed for osteochondral (OC) tissue engineering. Aqueous silk solution (16 wt.%) was used for preparation of the cartilage-like layer and, for generation of the silk/nano-CaP suspension and the bottom layer (CaP/Silk: 16 wt.%). The scaffolds were formed by using salt-leaching/lyophilization approach. The scanning electron microscopy revealed tha...
Custom-tailored tissue engineered polycaprolactone scaffolds for total disc rep...
Uden, S. van; Silva-Correia, Joana; Correlo, V. M.; Oliveira, J. M.; Reis, R. L.Degeneration of the intervertebral disc (IVD) represents a significant musculoskeletal disease burden. Tissue Engineering has been proposing several strategies comprising the use of biodegradable materials to prepare scaffolds that can present similar mechanical properties to native IVD tissues. However, this might be insufficient, since the patientâ s intervertebral space geometry must be replicated to allow t...
Current concepts and challenges in osteochondral tissue engineering and regener...
Yan, Leping; Oliveira, Joaquim M.; Oliveira, Ana Leite; Reis, R. L."Publication Date (Web): February 20, 2015"; In the last few years, great progress has been made to validate tissue engineering strategies in preclinical studies and clinical trials on the regeneration of osteochondral defects. In the preclinical studies, one of the dominant strategies comprises the development of biomimetic/bioactive scaffolds, which are used alone or incorporated with growth factors and/or st...
Bilayered silk/silk-nanoCaP scaffolds for osteochondral tissue engineering: in ...
Yan, Leping; Silva-Correia, Joana; Oliveira, Mariana B.; Vilela, Carlos; Pereira, H.; Sousa, R. A.; Mano, J. F.; Oliveira, A. L.; Oliveira, Joaquim M.Novel porous bilayered scaffolds, fully integrating a silk fibroin (SF) layer and a silk-nano calcium phosphate (Silk-NanoCaP) layer for osteochondral defect (OCD) regeneration were developed. Homogeneous porosity distribution was achieved in the scaffolds, with calcium phosphate phase only retained in the Silk-NanoCaP layer. The scaffold presented compressive moduli of 0.4 MPa in wet state. Rabbit bone marrow ...
Tumor growth suppression induced by biomimetic silk fibroin hydrogels
Leping, Yan; Silva-Correia, Joana; Ribeiro, Viviana Pinto; Gonçalves, Vera Mónica Miranda; Correia, Cristina; Morais, Alain José Silva; Sousa, R. A.Protein-based hydrogels with distinct conformations which enable encapsulation or differentiation of cells are of great interest in 3D cancer research models. Conformational changes may cause macroscopic shifts in the hydrogels, allowing for its use as biosensors and drug carriers. In depth knowledge on how 3D conformational changes in proteins may affect cell fate and tumor formation is required. Thus, this st...
Core-shell silk hydrogels with spatially tuned conformations as drug delivery s...
Yan, Leping; Oliveira, J. M.; Oliveira, A. L.; Reis, R. L.Hydrogels of spatially controlled physicochemical properties are appealing platforms for tissue engineering and drug delivery. In this study, core-shell silk fibroin (SF) hydrogels of spatially controlled conformation were developed. The core-shell structure in the hydrogels was formed by means of soaking the preformed (enzymatically crosslinked) random coil SF hydrogels in methanol. When increasing the methano...
Engineering nanoparticles for targeting rheumatoid arthritis: Past, present, an...
Oliveira, Isabel Maria Lopes Matos; Gonçalves, C.; Reis, R. L.; Oliveira, J. M.Rheumatoid arthritis (RA) is a chronic inflammatory disease characterized by synovial joint inflammation and cartilage and bone tissue destruction. Although there exist some treatment strategies for RA, they are not completely safe and effective. Therefore, it is important to develop and test new drugs for RA that specifically target inflamed/swollen joints and simultaneously attenuate other possible damages to...
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