Project/scholarship details
- Project/scholarship ID
120495
- Reference
PTDC/EBB-EBI/120495/2010
- FundRef URI
- Funder
FCT - Fundação para a Ciência e a Tecnologia, I.P.
- Funder's country
Portugal
- Funding program
5876-PPCDTI
- Funding amount
151,000.00 €
- Start date
2012-03-01
- End date
2015-08-31
Documents
Clinical Candida species co-infection and associated virulence
Alves, CarlosTese de doutoramento do Programa Doutoral em Engenharia Biomédica; The Candida genus consists of approximately 200 species of fungi and collectively represents a highly heterogenic group. Clinically, the most important specie is Candida albicans, an opportunistic fungal pathogen of humans that frequently causes superficial infections of oral and vaginal mucosal surfaces of debilitated individuals. This microorg...
Candida albicans promotes invasion and colonisation of Candida glabrata in a re...
Alves, Carlos; Wei, Xiao-Qing; Silva, Sónia Carina; Azeredo, Joana; Henriques, Mariana; Williams, DavidThe principal aim of this study was to investigate the in vitro co-infection of a reconstituted human vaginal epithelium (RHVE) by Candida albicans and Candida glabrata. Methods The ability of both species to invade and colonise the RHVE was examined using species-specific peptide nucleic acid (PNA) probe hybridisation, confocal laser scanning microscopy (CLSM) and a novel qRT-PCR protocol for Candida quantific...
Influence of glucose concentration on the structure and quantity of biofilms fo...
Pereira, Leonel; Silva, Sónia Carina; Ribeiro, Bruno; Henriques, Mariana; Azeredo, JoanaCandida parapsilosis is nowadays an emerging opportunistic pathogen and its increasing incidence is part related to the capacity to produce biofilm. In addition, one of the most important C. parapsilosis pathogenic risk factors includes the organisms\textquoteright selective growth capabilities in hyper alimentation solutions. Thus, in this study, we investigated the role of glucose in C. parapsilosis biofilm m...
Novel strategies to fight Candida species infection
Rodrigues, E.; Silva, Sónia Carina; Azeredo, Joana; Henriques, MarianaIn recent years, there has been a significant increase in the incidence of human fungal infections. The increase in cases of infection caused by Candida species, and the consequent excessive use of antimicrobials, has favored the emergence of resistance to conventional antifungal agents over the past decades. Consequently, Candida infections morbidity and mortality are also increasing. Therefore, new approaches...
Vulvovaginal candidiasis: Epidemiology, microbiology and risk factors
Gonçalves, B.; Ferreira, C.; Alves, Carlos; Henriques, Mariana; Azeredo, Joana; Silva, Sónia CarinaVulvovaginal candidiasis (VVC) is an infection caused by Candida species that affects millions of women every year. Although Candida albicans is the main cause of VVC, the identification of non-Candida albicans Candida (NCAC) species, especially Candida glabrata, as the cause of this infection, appears to be increasing. The development of VVC is usually attributed to the disturbance of the balance between Candi...
Candida tropicalis biofilm and human epithelium invasion is highly influenced b...
Ferreira, C.; Gonçalves, Bruna; Boas, Diana Patrícia Andrade Vilas; Oliveira, Hugo; Henriques, Mariana; Azeredo, Joana; Silva, Sónia CarinaObjective: The main goal of this study was to investigate the role of pH on Candida tropicalis virulence determinants, namely the ability to form biofilms and to colonize/invade reconstituted human vaginal epithelia. Methods: Biofilm formation was evaluated by enumeration of cultivable cells, total biomass quantification and structural analysis by scanning electron microscopy and confocal laser scanning microsc...
Candida species biofilms' antifungal resistance
Silva, Sónia Carina; Rodrigues, Célia Fortuna; Araújo, Daniela; Rodrigues, Maria Elisa Costa; Henriques, MarianaCandida infections (candidiasis) are the most prevalent opportunistic fungal infection on humans and, as such, a major public health problem. In recent decades, candidiasis has been associated to Candida species other than Candida albicans. Moreover, biofilms have been considered the most prevalent growth form of Candida cells and a strong causative agent of the intensification of antifungal resistance. As yet,...
The CgHaa1-regulon mediates response and tolerance to acetic acid stress in the...
Bernardo, Ruben T.; Cunha, Diana V.; Wang, Can; Pereira, L. P.; Silva, Sónia Carina; Salazar, Sara B.; Schröder, Markus S.; Okamoto, MichiyoTo thrive in the acidic vaginal tract C. glabrata has to cope with high concentrations of acetic acid. The mechanisms underlying C. glabrata tolerance to acetic acid at low pH remain largely uncharacterized. In this work it is demonstrated the essential role of the CgHaa1 transcription factor (encoded by ORF CAGL0L09339g) in the response and tolerance of C. glabrata to acetic acid. Transcriptomic analysis showe...
Portrait of Candida species biofilm regulatory network genes
Araújo, Daniela; Henriques, Mariana; Silva, Sónia CarinaMost cases of candidiasis have been attributed to Candida albicans, but Candida glabrata, Candida parapsilosis and Candida tropicalis, designated as non-C. albicans Candida (NCAC), have been identified as frequent human pathogens. Moreover, Candida biofilms are an escalating clinical problem associated with significant rates of mortality. Biofilms have distinct developmental phases, including adhesion/colonisat...
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