Project/scholarship details
- Project/scholarship ID
126270
- Reference
RECI/BBB-EBI/0179/2012
- FundRef URI
- Funder
FCT - Fundação para a Ciência e a Tecnologia, I.P.
- Funder's country
Portugal
- Funding program
5876-PPCDTI
- Funding amount
338,852.00 €
- Start date
2013-01-01
- End date
2015-12-31
Documents
On the independence of hydrogen production from methanogenic suppressor in oliv...
Gonçalves, Marta R.; Costa, J. C.; Pereira, M. A.; Abreu, A. A.; Alves, M. M.Anaerobic degradation of olive mill wastewater (OMW) at concentrations ranging from 2 to 100 g/L of chemical oxygen demand (COD) was assessed in batch assays. Methane was the main final product obtained for the lower concentrations tested. For 25 g COD/L, H2 was temporarily produced, albeit H2 depletion occurred, likely due to homoacetogenesis, since acetate was formed concomitantly. Hydrogen was produced and a...
Design of experiments to assess pre-treatment and co-digestion strategies that ...
Oliveira, J. V. M.; Alves, M. M.; Costa, J. C.A design of experiments was applied to evaluate different strategies to enhance the methane yield of macroalgae Gracilaria vermiculophylla. Biochemical Methane Potential (BMP) of G. vermiculophylla after physical pre-treatment (washing and maceration) reached 481 ± 9 L CH4 kg−1 VS, corresponding to a methane yield of 79 ± 2%. No significant effects were achieved in the BMP after thermochemical pre-treatment, al...
Cre-loxP-based system for removal and reuse of selection markers in Ashbya goss...
Aguiar, Tatiana Quinta; Dinis, Cláudia; Domingues, LucíliaThe filamentous ascomycete Ashbya gossypii is amenable to genetic manipulation and is an excellent model system for studying eukaryotic cell biology. However, the number of selection markers in current use for both targeted gene integration and disruption in this fungus are very limited. Therefore, the Cre-loxP recombination system was adapted for use in A. gossypii and its effectiveness in recycling marker gen...
Investigation of protein secretion and secretion stress in Ashbya gossypii
Aguiar, Tatiana Quinta; Ribeiro, Orquídea; Arvas, Mikko; Wiebe, Marilyn G.; Penttilä, Merja; Domingues, LucíliaBackground: Ashbya gossypii is a filamentous Saccharomycete used for the industrial production of riboflavin that has been recently explored as a host system for recombinant protein production. To gain insight into the protein secretory pathway of this biotechnologically relevant fungus, we undertook genome-wide analyses to explore its secretome and its transcriptional responses to protein secretion stress. Res...
Modulation of crude glycerol fermentation byClostridium pasteurianum DSM 525 to...
Gallardo, R.; Alves, M. M.; Rodrigues, L. R.High production yields and productivities are requisites for the development of an industrial butanol production process based on biodiesel-derived crude glycerol. However, impurities present in this substrate and/or the concentration of glycerol itself can affect the microbial metabolism. In this work, the effect of crude glycerol concentration on the production of butanol and 1,3-propanediol (1,3-PDO) by Clos...
Gardnerella vaginalis outcompetes 29 other bacterial species isolated from BV p...
Alves, Patrícia Maria; Castro, J.; Sousa, Cármen; Cereija, Tatiana Barros Reis; Cerca, NunoDespite the worldwide prevalence of bacterial vaginosis (BV), its etiology is still unknown. Although BV has been associated with the presence of biofilm, the ability of BV-associated bacteria to form biofilms is still largely unknown. Here, we isolated 30 BV-associated species and characterized their virulence, using an in vitro biofilm formation model. Our data suggests that Gardnerella vaginalis had the high...
Co-digestion of Sargassum sp. with glycerol and waste frying oil following a de...
Oliveira, João Vítor; Alves, M. M.; Costa, J. C.A response surface methodology was adopted to assess the optimal conditions for methane production from the macroalgae Sargassum sp. co-digested with glycerol (Gly) and waste frying oil (WFO). Three variables were tested: % total solids of algae (%TSSargassum sp.), co-substrate concentration (gGly/WFO L-1); and, co-substrate type (Gly or WFO). The Biochemical Methane Potential (BMP) of Sargassum sp. was 181±1 L...
Co-digestion of Sargassum sp. with glycerol and waste frying oil: optimization ...
Oliveira, João Vítor; Alves, M. M.; Costa, J. C.A response surface methodology was adopted to assess the optimal conditions for methane production from the macroalgae Sargassum sp. Three variables were tested: % total solids of algae (%TSsargassum); co-substrate concentration (gwaste L-1); and, co-substrate type (glycerol or waste frying oil (WFO)). The Biochemical Methane Potential (BMP) of Sargassum sp. was 300 ± 3 L CH4 kg-1 COD. The co-digestion with gly...
Effects of biosurfactants on the viability and proliferation of human breast ca...
Duarte, Cristina; Gudiña, Eduardo J.; Lima, Cristóvão F.; Rodrigues, L. R.Biosurfactants are molecules with surface activity produced by microorganisms that can be used in many biomedical applications. The anti-tumour potential of these molecules is being studied, although results are still scarce and few data are available regarding the mechanisms underlying such activity. In this work, the anti-tumour activity of a surfactin produced by Bacillus subtilis 573 and a glycoprotein (Bio...
Pseudomonas aeruginosa diversification during infection development in cystic f...
Sousa, A. M.; Pereira, Maria OlíviaPseudomonas aeruginosa is the most prevalent pathogen of cystic fibrosis (CF) lung disease. Its long persistence in CF airways is associated with sophisticated mechanisms of adaptation, including biofilm formation, resistance to antibiotics, hypermutability and customized pathogenicity in which virulence factors are expressed according the infection stage. CF adaptation is triggered by high selective pressure o...
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