Project/scholarship details
- Project/scholarship ID
127180
- Reference
PTDC/CTM-BIO/1814/2012
- FundRef URI
- Funder
FCT - Fundação para a Ciência e a Tecnologia, I.P.
- Funder's country
Portugal
- Funding program
5876-PPCDTI
- Funding amount
199,948.00 €
- Start date
2013-01-01
- End date
2015-03-31
Documents
Development of natural-based hydrogel particles using a biomimetic methodology
Lima, Ana Catarina CardosoTese de doutoramento em Bioengenharia; Superhydrophobic (SH) surfaces have been greatly explored in the biomedical field. Such surfaces are inspired by the repellent properties of natural structures. The most well known example is the lotus leaf, which has the capacity to repeal the water droplets due to the presence of micro/nano topographical features and the low surface energy. One particular application of ...
Free and copolymerized gamma-cyclodextrins regulate performance of dexamethason...
Lima, A. C.; Puga, Ana M.; Mano, J. F.; Concheiro, Angel; Alvarez-Lorenzo, CarmenPolymeric particles acting as source of biological cues to promote tissue regeneration are currently an interesting topic in bone tissue engineering research. In this study, microspheres of dextran-methacrylated (dextran-MA) and γ-cyclodextrins (γ-CD) for the delivery of osteogenic agents were prepared by means of photopolymerization on biomimetic superhydrophobic surfaces. The effects of the incorporation of t...
Superhydrophobic chips for cell spheroids high-throughput generation and drug s...
Oliveira, Mariana B.; Neto, Ana I.; Correia, Clara R.; Rial-Hermida, Maria Isabel; Álvarez-Lorenzo, Carmen; Mano, J. F.We suggest the use of biomimetic superhydrophobic patterned chips produced by a bench-top methodology as low-cost and waste-free platforms for the production of arrays of cell spheroids/microtissues by the hanging drop methodology. Cell spheroids have a wide range of applications in biotechnology fields. For drug screening, they allow studying 3D models in structures resembling real living tissues/tumors. In ti...
Enhanced cell affinity of chitosan membranes mediated by superficial cross-link...
Rodríguez-Velázquez, Eustolia; Silva, Maite; Taboada, Pablo; Mano, J. F.; Suárez-Quintanilla, D.; Alatorre-Meda, ManuelIt is well accepted that the surface modification of biomaterials can improve their biocompatibility. In this context, techniques like ion etching, plasma-mediated chemical functionalization, electrospinning, and contact microprinting have successfully been employed to promote the cell adhesion and proliferation of chitosan (CH) substrates. However, they prove to be time-consuming, highly dependent on environme...
Biomimetic miniaturized platform able to sustain arrays of liquid droplets for ...
Neto, Ana I.; Correia, Clara R.; Custódio, Catarina A.; Mano, J. F.The development of high-throughput and combinatorial technologies is helping to speed up research that is applicable in many areas of chemistry, engineering, and biology. A new model is proposed for flat devices for the high-throughput screening of accelerated evaluations of multiplexed processes and reactions taking place in aqueous-based environments. Superhydrophobic (SH) biomimetic surfaces based on the so-...
Molecular interactions driving the layer-by-layer assembly of multilayers
Borges, J.; Mano, J. F.This article presents an overview of the different types of intermolecular interactions behind the fabrication of multilayer assemblies using the layer-by-layer (LbL) assembly approach. It comments on the potential impact of each type of intermolecular interaction and materials assembled through them on the development of advanced functional systems or devices for several emerging applications. The discussion b...
Sequential ionic and thermogelation of chitosan spherical hydrogels prepared us...
Lima, A. C.; Correia, Clara R.; Oliveira, Mariana B.; Mano, J. F.Chitosan is soluble in acidic media, which makes it incompatible for the encapsulation of cells and pH-sensitive molecules. In this work, a mild chitosan-based system with two sequential gelation steps is proposed, where the model drug dexamethasone and L929 cells are immobilized inside hydrogel beads. Superhydrophobic surfaces were used to produce the spherical hydrogel particles that provided favorable condit...
Biocompatible polymeric microparticles produced by a simple biomimetic approach
Costa, A. M. S.; Alatorre-Meda, Manuel; Oliveira, N. M.; Mano, J. F.The use of superhydrophobic surfaces to produce polymeric particles proves to be biologically friendly since it entails the pipetting and subsequent cross-linking of polymeric solutions under mild experimental conditions. Moreover, it renders encapsulation efficiencies of ∼100%. However, the obtained particles are 1 to 2 mm in size, hindering to a large extent their application in clinical trials. Improving on ...
Biomimetic superhydrophobic surfaces patterned with wettable spots as implantab...
Oliveira, Mariana Braga; Ribeiro, Maximiano P.; Miguel, Sónia P.; Neto, Ana I.; Coutinho, Paula; Correia, Ilídio J.; Mano, J. F."Tissue engineering: part A", vol. 21, suppl. 1 (2015); Flat patterned superhydrophobic films are herein reported as implantable platforms containing arrays of miniaturized porous scaffolds for the high-throughput in vivo study of biomaterials. Highthroughput platforms are powerful platforms to detect hit conditions showing promising properties. However, high-content analysis of biomaterials has been mostly lim...
Assembly of cell-laden hydrogel fiber into non-liquefied and liquefied 3D spira...
Praveen, S.; Oliveira, Sara M.; Borges, J.; Mano, J. F.In this work, three-dimensional (3D) self-sustaining, spiral-shaped constructs were produced through a combination of ionotropic gelation, to form cell-encapsulated alginate fibers, and a perfusion-based layer-by-layer (LbL) technique. Single fibers were assembled over cylindrical molds by reeling to form spiral shapes, both having different geometries and sizes. An uninterrupted nanometric multilayer coating p...
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