Project/scholarship details
- Project/scholarship ID
129208
- Reference
PTDC/AGR-FOR/3090/2012
- FundRef URI
- Funder
FCT - Fundação para a Ciência e a Tecnologia, I.P.
- Funder's country
Portugal
- Funding program
5876-PPCDTI
- Funding amount
102,348.00 €
- Start date
2013-07-01
- End date
2015-09-30
Documents
Engineering yeast tolerance to inhibitory lignocellulosic biomass
Cunha, Joana Filipa Torres Pinheiro; Aguiar, Tatiana Quinta; Mendes, D.; Pereira, Francisco B.; Domingues, LucíliaIn recent years the necessity for biotechnological manufacturing based on lignocellulosic feedstocks has become evident. However, the pre-treatment step in the production of lignocellulosic bioethanol leads to the accumulation of inhibitory byproducts. Robust second generation bioethanol processes require microorganisms able to ferment these inhibitory lignocellulosic hydrolysates. Significant progress has been...
Production of recombinant carbohydrate-binding modules in Pichia pastoris and t...
Sepúlveda, Maria Goreti CastroDissertação de mestrado em Bioengenharia; Enzymes have been widely applied in the paper industry for the modification of pulp and paper properties. Although they have the potential to replace hazardous chemicals, their use in the hydrolysis of polysaccharides reduces both fiber strength and mass. To overcome these negative effects, the potential use of recombinant carbohydrate-binding modules (CBMs) in papermak...
Recombinant production of plant lectins in microbial systems for biomedical app...
Oliveira, Carla Cristina Marques de; Teixeira, J. A.; Domingues, LucíliaFrutalin is a homotetrameric partly-glycosylated alpha-D-galactose-binding lectin of biomedical interest from Artocarpus incisa (breadfruit) seeds, belonging to the jacalin-related lectins family. As other plant lectins, frutalin is a heterogeneous mixture of several isoforms possibly with distinct biological activities. The main problem of using such lectins as biomedical tools is that “batch-to-batch” variati...
Bioethanol: robust production strains for process intensification
Pereira, Luciano Francisco BorgesTese de Doutoramento em Engenharia Química e Biológica.; The production of bioethanol operating at high substrate loading improves the overall process productivity and reduces initial capital investment and water consumption comparing to processing at normal gravity. However, there are some inhibition issues that become more severe under these operation conditions. This thesis focused on the development of effi...
Effect of hot calendering on physical properties and water vapor transfer resis...
Costa, V. L. D.; Costa, A. P.; Amaral, M. E.; Oliveira, Carla; Gama, F. M.; Dourado, Fernando; Simões, R. M.This work investigates the effect of hot calendering on bacterial cellulose (BC) films properties, aiming the achievement of good transparency and barrier property. A comparison was made using vegetal cellulose (VC) films on a similar basis weight of around 40 g.m-2. The optical-structural, mechanical and barrier property of BC films were studied and compared with those of highly beaten VC films. The Youngs mod...
Valorizing recycled paper sludge by a bioethanol production process with cellul...
Gomes, Daniel Gonçalves; Domingues, Lucília; Gama, F. M.The feasibility of cellulase recycling in the scope of bioethanol production from recycled paper sludge (RPS), an inexpensive byproduct with around 39% of carbohydrates, is analyzed. RPS was easily converted and fermented by enzymes and cells, respectively. Final enzyme partition between solid and liquid phases was investigated, the solid-bound enzymes being efficiently recovered by alkaline washing. RPS hydrol...
Synthesis of fusion genes for cloning by megaprimer-based PCR
Aguiar, Tatiana Quinta; Oliveira, Carla Cristina Marques de; Domingues, LucíliaThe polymerase chain reaction (PCR) is the technique of choice used to obtain DNA for cloning, because it rapidly provides high amounts of desired DNA fragments and allows the easy introduction of extremities adequate for enzyme restriction or homologous recombination, and of artificial, native, or modified sequence elements for specific applications. In this context, the use of megaprimer-based PCR strategies ...
Enhancing the enzymatic saccharification of whole slurry from autohydrolyzed Eu...
Oliveira, Carla; Romaní, Aloia; Gomes, Daniel G.; Cunha, Joana T.; Gama, F. M.; Domingues, LucíliaMicrobiotec'17 - Congress of Microbiology and Biotechnology 2017; Background: Lignocellulosic biomass has a recognised potential as a sustainable platform for the production of biofuels and other biochemicals. However, lignin residues and other inhibitory compounds resulting from lignocellulosics pretreatment affect the digestibility of resulting whole slurries. The addition of synergistic proteins that can coo...
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