Project/scholarship details


  • Funder

    FCT - Fundação para a Ciência e a Tecnologia, I.P.

  • Funder's country

    Portugal

  • Funding program

    5876-PPCDTI

  • Funding amount

    43,590.00 €

  • Start date

    2014-04-07

  • End date

    2015-09-30

Documents


Pydna: a simulation and documentation tool for DNA assembly strategies using py...

Pereira, Filipa Alexandra Barroso; Azevedo, Flavio; Carvalho, Angela; Ribeiro, Gabriela F.; Budde, Mark W.; Johansson, Björn

Background: Recent advances in synthetic biology have provided tools to efficiently construct complex DNA molecules which are an important part of many molecular biology and biotechnology projects. The planning of such constructs has traditionally been done manually using a DNA sequence editor which becomes error-prone as scale and complexity of the construction increase. A human-readable formal description of ...


Engineering a commercial cold-adapted xylanase for improved acid pH stability

Barroca, Mário Jorge Faria; Santos, Gustavo de Almeida; Oliveira, Bruno; Veloso, Paulo; Silva, Diogo; Johansson, Björn; Collins, Tony

[Excerto] Xylanases (EC 3.2.1.8) are O-glycoside hydrolases which randomly cleave the internal beta-1,4D-xylosidic linkages of the complex plant heteropolysaccharide xylan. pXyl is a cold-adapted xylanase characterised by a high activity and specificity for xylan and is currently being successfully commercialised worldwide for baking applications. It also has high potential for application in the beverages indu...


Antibiotic free selection for the high level biosynthesis of a silk-elastin-lik...

Barroca, Mário Jorge Faria; Rodrigues, Paulo; Sobral, Rómulo Sacramento; Costa, Maria Manuela Ribeiro; Chaves, Susana R.; Machado, Raul

Silk-elastin-like proteins (SELPs) are a family of genetically engineered recombinant protein polymers exhibiting mechanical and biological properties suited for a wide range of applications in the biomedicine and materials fields. They are being explored as the next generation of biomaterials but low productivities and use of antibiotics during production undermine their economic viability and safety. We have ...


Protein engineering for low pH stability: the role of exposed surface residues

Barroca, Mário Jorge Faria; Santos, Gustavo de Almeida; Oliveira, Bruno; Pacheco, M. P.; Veloso, Paulo; Silva, Diogo; Collins, Tony

This work was financed by national funds from the Fundação para a Ciência e Tecnologia through project EXPL/BBB-BIO/1772/2013 and through strategic funding via UID/BIA/04050/2013.; info:eu-repo/semantics/publishedVersion


Activity-stability relationships revisited in blue oxidases catalyzing electron...

Roulling, Frédéric; Godin, Amandine; Cipolla, Alexandre; Collins, Tony; Miyazaki, Kentaro; Feller, Georges

The online version of this article (doi:10.1007/s00792-016-0851-9) contains supplementary material, which is available to authorized users.; Cuproxidases are a subset of the blue multicopper oxidases that catalyze the oxidation of toxic Cu(I) ions into less harmful Cu(II) in the bacterial periplasm. Cuproxidases from psychrophilic, mesophilic, and thermophilic bacteria display the canonical features of temperat...


Biotechnological aspects of cold-active enzymes

Barroca, Mário Jorge Faria; Santos, Gustavo; Gerday, Charles; Collins, Tony

Cold-adapted enzymes produced by organisms inhabiting permanently low temperature environments are typically characterized by a high activity at low to moderate temperatures and a poor thermal stability. Such characteristics make these enzymes highly attractive for various applications where they can enable more efficient, cost-effective, and environmentally friendlier processes than higher temperature-adapted ...


Deciphering the factors defining the pH-dependence of a commercial glycoside hy...

Barroca, Mário Jorge Faria; Santos, Gustavo; Johansson, Björn; Gillotin, Florian; Feller, Georges; Collins, Tony

A prerequisite to the use of any enzyme in any industrial process is an understanding of its activity and stability under process conditions. Glycoside hydrolase family 8 enzymes include many important biotechnological biocatalysts yet little is known of the performance of these with respect to pH. A better understanding of this parameter and its relationship to structure and function in these enzymes will allo...


Enzyme catalysis in psychrophiles

Collins, Tony; Gerday, Charles

Cold-active enzymes are produced by organisms, known as psychrophiles, adapted to permanently cold habitats. Low temperatures have an exponential deleterious effct on reaction rates, and thus psychrophilic enzymes have to be adapted to secure appropriate reaction rates in their environment. These enzymes have a high specific activity at low temperatures, in any case higher than that of their mesophilic and ther...

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