Project/scholarship details
- Project/scholarship ID
135240
- Reference
EXPL/EMS-SIS/2215/2013
- FundRef URI
- Funder
FCT - Fundação para a Ciência e a Tecnologia, I.P.
- Funder's country
Portugal
- Funding program
5876-PPCDTI
- Funding amount
46,500.00 €
- Start date
2014-03-17
- End date
2015-08-16
Documents
Simple methodology for the quantitative analysis of fatty acids in human red bl...
Rodrigues, Raquel O.; Costa, Helena; Lima, Rui Alberto Madeira Macedo; Amaral, Joana S.In the last years, there has been an increasing interest in evaluating possible relations between fatty acid (FA) patterns and the risk for chronic diseases. Due to the long life span (120 days) of red blood cells (RBCs), their FA profile reflects a longer term dietary intake and was recently suggested to be used as an appropriate biomarker to investigate correlations between FA metabolism and diseases. Therefo...
A rapid and low-cost nonlithographic method to fabricate biomedical microdevice...
Pinto, Elmano; Faustino, Vera; Rodrigues, Raquel O.; Pinho, Diana; Garcia, Valdemar; Miranda, João M.; Lima, Rui Alberto Madeira MacedoMicrofluidic devices are electrical/mechanical systems that offer the ability to work with minimal sample volumes, short reactions times, and have the possibility to perform massive parallel operations. An important application of microfluidics is blood rheology in microdevices, which has played a key role in recent developments of lab-on-chip devices for blood sampling and analysis. The most popular and tradit...
A simple microfluidic device for the deformability assessment of blood cells in...
Rodrigues, Raquel O.; Pinho, Diana; Faustino, Vera; Lima, Rui Alberto Madeira MacedoBlood flow presents several interesting phenomena in microcirculation that can be used to develop microfluidic devices capable to promote blood cells separation and analysis in continuous flow. In the last decade there have been numerous microfluidic studies focused on the deformation of red blood cells (RBCs) flowing through geometries mimicking microvessels. In contrast, studies focusing on the deformation of...
Blood flow visualization and measurements in microfluidic devices fabricated by...
Singhal, Jaron; Pinho, Diana; Lopes, Raquel; Sousa, Patricia C.; Garcia, Valdemar; Schütte, Helmut; Lima, Rui Alberto Madeira Macedo; Gassmann, StefanThe most common and used technique to produce microfluidic devices for biomedical applications is the soft-lithography. However, this is a high cost and time-consuming technique. Recently, manufacturers were able to produce milling tools smaller than 100 m and consequently have promoted the ability of the micromilling machines to fabricate microfluidic devices capable of performing cell separation. In this work...
Generation of micro-sized PDMS particles by a flow focusing technique for biomi...
Muñoz-Sánchez, B. N.; Silva, S. F.; Pinho, Diana; Vega, E. J.; Lima, Rui Alberto Madeira MacedoPolydimethylsiloxane (PDMS), due to its remarkable properties, is one of the most widely used polymers in many industrial and medical applications. In this work, a technique based on a flow focusing technique is used to produce PDMS spherical particles with sizes of a few microns. PDMS precursor is injected through a hypodermic needle to form a film/reservoir over the needle's outer surface. This film flows tow...
Particulate blood analogues reproducing the erythrocytes cell-free layer in a m...
Calejo, Joana; Pinho, Diana; Galindo-Rosales, Francisco J.; Lima, Rui Alberto Madeira Macedo; Campo-Deaño, LauraThe interest in the development of blood analogues has been increasing recently as a consequence of the increment in the number of experimental hemodynamic studies and the difficulties associated with the manipulation of real blood in vitro because of ethical, economical or hazardous issues. Although one-phase Newtonian and non-Newtonian blood analogues can be found in the literature, there are very few studies...
Red blood cells radial dispersion in blood flowing through microchannels: the r...
Pinho, Diana; Rodrigues, Raquel O.; Faustino, Vera; Yaginuma, T.; Exposto, José; Lima, Rui Alberto Madeira MacedoThe behavior of suspensions of individual blood cells, such as red blood cells (RBCs), flowing through microvessels and microfluidic systems depend strongly on the hematocrit (Hct), microvessel topology and cell properties. Although it is well known that blood rheological properties are temperature dependent, to the best of our knowledge no work has studied the role of the temperature on the RBCs dispersion. A ...
Microbubble moving in blood flow in microchannels: effect on the cell-free laye...
Bento, D.; Sousa, Lúcia; Yaginuma, T.; Garcia, Valdemar; Lima, Rui Alberto Madeira Macedo; Miranda, João M.Gas embolisms can hinder blood flow and lead to occlusion of the vessels and ischemia. Bubbles in microvessels circulate as tubular bubbles (Taylor bubbles) and can be trapped, blocking the normal flow of blood. To understand how Taylor bubbles flow in microcirculation, in particular, how bubbles disturb the blood flow at the scale of blood cells, experiments were performed in microchannels at a low Capillary n...
In vitro particulate analogue fluids for experimental studies of rheological an...
Pinho, Diana; Campo-Deaño, Laura; Lima, Rui Alberto Madeira Macedo; Pinho, Fernando T.Suspensions of healthy and pathological red blood cells (RBC) flowing in microfluidic devices are frequently used to performin vitroblood experiments for a better understanding of human microcirculation hemodynamic phenomena. This work reports the development of particulate viscoelastic analogue fluids able to mimic the rheological and hemorheological behavior of pathological RBC suspensions flowing in microflu...
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