Project/scholarship details
- Project/scholarship ID
66151
- Reference
PTDC/BIO/66151/2006
- FundRef URI
- Funder
FCT - Fundação para a Ciência e a Tecnologia, I.P.
- Funder's country
Portugal
- Funding program
5876-PPCDTI
- Funding amount
141,000.00 €
- Start date
2007-09-01
- End date
2010-11-30
Documents
Fermentation of high concentrations of lactose to ethanol by engineered floccul...
Guimarães, Pedro M. R.; Teixeira, J. A.; Domingues, LucíliaThe development of microorganims that efficiently ferment lactose has a high biotechnological interest, particularly for cheese whey bioremediation processes with simultaneous bio-ethanol production. The lactose fermentation performance of a recombinant Saccharomyces cerevisiae flocculent strain was evaluated. The yeast consumed rapidly and completely lactose concentrations up to 150 g l-1 in either well- or mi...
Selection of Saccharomyces cerevisiae strains for efficient very high gravity b...
Pereira, Francisco B.; Guimarães, Pedro M. R.; Teixeira, J. A.; Domingues, LucíliaAn optimized very high gravity (VHG) glucose medium supplemented with low cost nutrient sources was used to evaluate bio-ethanol production by 11 Saccharomyces cerevisiae strains. The industrial strains PE-2 and CA1185 exhibited the best overall fermentation performance, producing an ethanol titre of 19.2% (v/v) corresponding to a batch productivity of 2.5 g l-1 h-1, while the best laboratory strain (CEN.PK 113...
Fermentation of deproteinized cheese whey powder solutions to ethanol by engine...
Silva, Ana Carina; Guimarães, Pedro M. R.; Teixeira, J. A.; Domingues, LucíliaThe lactose in cheese whey is an interesting substrate for the production of bulk commodities such as bio-ethanol, due to the large amounts of whey surplus generated globally. In this work, we studied the performance of a recombinant Saccharomyces cerevisiae strain expressing the lactose permease and intracellular ß-galactosidase from Kluyveromyces lactis in fermentations of deproteinized concentrated cheese wh...
Optimization of low-cost medium for very high gravity ethanol fermentations by ...
Pereira, Francisco B.; Guimarães, Pedro M. R.; Teixeira, J. A.; Domingues, LucíliaStatistical experimental designs were used to develop a medium based on corn steep liquor (CSL) and other low-cost nutrient sources for high-performance very high gravity (VHG) ethanol fermentations by Saccharomyces cerevisiae. The critical nutrients were initially selected according to a Plackett–Burman design and the optimized medium composition (44.3 g/L CSL; 2.3 g/L urea; 3.8 g/L MgSO4·7H2O; 0.03 g/L CuSO4·...
Fermentation of lactose to bio-ethanol by yeasts as part of integrated solution...
Guimarães, Pedro M. R.; Teixeira, J. A.; Domingues, LucíliaCheese whey, the main dairy by-product, is increasingly recognized as a source of many bioactive valuable compounds. Nevertheless, the most abundant component in whey is lactose (ca. 5% w/v), which represents a significant environmental problem. Due to the large lactose surplus generated, its conversion to bio-ethanol has long been considered as a possible solution for whey bioremediation. In this review, ferme...
Robust industrial Saccharomyces cerevisiae strains for very high gravity bio-et...
Pereira, Francisco B.; Guimarães, Pedro M. R.; Teixeira, J. A.; Domingues, LucíliaThe application and physiological background of two industrial Saccharomyces cerevisiae strains, isolated from harsh industrial environments, were studied in Very High Gravity (VHG) bio-ethanol fermentations. VHG laboratory fermentations, mimicking industrially relevant conditions, were performed with PE-2 and CA1185 industrial strains and the CEN.PK113-7D laboratory strain. The industrial isolates produced rem...
Identification of candidate genes for yeast engineering to improve bioethanol p...
Pereira, Francisco B.; Guimarães, Pedro M. R.; Gomes, Daniel Gonçalves; Mira, Nuno P.; Teixeira, Miguel C.; Sá-Correia, Isabel; Domingues, LucíliaBackground: The optimization of industrial bioethanol production will depend on the rational design and manipulation of industrial strains to improve their robustness against the many stress factors affecting their performance during very high gravity (VHG) or lignocellulosic fermentations. In this study, a set of Saccharomyces cerevisiae genes found, through genome-wide screenings, to confer resistance to the ...
Bioethanol: robust production strains for process intensification
Pereira, Luciano Francisco BorgesTese de Doutoramento em Engenharia Química e Biológica.; The production of bioethanol operating at high substrate loading improves the overall process productivity and reduces initial capital investment and water consumption comparing to processing at normal gravity. However, there are some inhibition issues that become more severe under these operation conditions. This thesis focused on the development of effi...
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