Project/scholarship details
- Project/scholarship ID
68393
- Reference
PTDC/AMB/68393/2006
- FundRef URI
- Funder
FCT - Fundação para a Ciência e a Tecnologia, I.P.
- Funder's country
Portugal
- Funding program
5876-PPCDTI
- Funding amount
104,240.00 €
- Start date
2008-04-01
- End date
2011-12-31
Documents
Ecological study of the filamentous bacteria in the mixed liquor of activated s...
Santos, Liliana; Neto, Marta Martins; Mina, Isabel Aguiar Pinto; Lima, Nelson; Mota, M.; Nicolau, AnaFundação para a Ciência e a Tecnologia (FCT) - PTDC/AMB/68393/2006, SFRH/BD/64848/2009, SFRH/BD/61282/2009
Candida tropicalis biofilms : artificial urine, urinary catheters and flow model
Negri, Melyssa; Silva, Sónia Carina; Henriques, Mariana; Azeredo, Joana; Svidzinski, Terezinha; Oliveira, RosárioAdhesion to medical devices and biofilm formation are considered important virulence factors of Candida tropicalis. This work aimed to use artificial urine (AU) and urinary catheters, under flow conditions, for the study of C. tropicalis biofilms. Adhesion and biofilm formation on silicone and latex urinary catheters was quantified by crystal violet staining and colony forming units determination. Candida surfa...
New insights on the roles of bacteria and protozoa in activated-sludge processes
Ferreira, Vânia; Santos, Liliana; Neto, Marta Martins; Fernandes, José Manuel da Silva; Pereira, M. A.; Lourenço, Anália; Mota, M.; Lima, NelsonWastewater treatment is one of the most important biotechnological processes in the world. Nevertheless, the highly complex microbial communities in wastewater treatment plants (WWTP) are barely understood, remaining, most of the time, as a “black box”. Even though many microbiological methods are available to study the activated-sludge communities, many recent questions on the role of a significant number of m...
Adaptive response of single and binary Pseudomonas aeruginosa and Escherichia c...
Machado, Idalina; Lopes, Susana Patrícia; Sousa, Ana Margarida; Pereira, M. O.The main goal of this work was to examine whether the continuous exposure of single and binary P. aeruginosa and E. coli biofilms to sub-lethal benzalkonium chloride (BC) doses can induce adaptive response of bacteria. Biofilms were formed during 24 h and then put continuously in contact with BC for more 5 days. The six-day-old adapted biofilms were then submitted to BC challenge, characterized and inspected by...
Insights into Candida tropicalis nosocomial infections and virulence factors
Negri, M.; Silva, Sónia Carina; Henriques, Mariana; Oliveira, RosárioCandida tropicalis is considered the first or the second non-Candida albicans Candida (NCAC) species most frequently isolated from candidosis, mainly in patients admitted in intensive care units (ICUs), especially with cancer, requiring prolonged catheterization, or receiving broad-spectrum antibiotics. The proportion of candiduria and candidemia caused by C. tropicalis varies widely with geographical area and ...
Transformation of Escherichia coli JM109 using pUC19 by the Yoshida effect
Mendes, Gabriel; Vieira, Pedro S.; Lanceros-Méndez, S.; Kluskens, Leon; Mota, M.Transformation of non-competent Escherichia coli JM109 was accomplished using pUC19 as donor plasmid and sepiolite as the acicular material to promote cell piercing via application of friction with a polystyrene stick or a magnetic bar on the surface of a hydrogel containing agar. An automatic spreading setup was built with a conventional stirring plate and compared to manual spreading. Several parameters were ...
The first Paenibacillus larvae bacteriophage endolysin (PlyPl23) with high pote...
Oliveira, A.; Leite, Marta; Kluskens, Leon; Santos, Sílvio Roberto Branco; Melo, Luís D. R.; Azeredo, JoanaEndolysins, which are peptidoglycan-degrading enzymes expressed during the terminal stage of the reproduction cycle of bacteriophages, have great potential to control Gram-pos- itive pathogens. This work describes the characterization of a novel endolysin (PlyPl23) encoded on the genome of Paenibacillus larvae phage phiIBB_Pl23 with high potential to control American foulbrood. This bacterial disease, caused by...
Candida tropicalis biofilms: biomass, metabolic activity and secreted aspartyl ...
Grassi, M.; Silva, Sónia Carina; Capoci, I. R. G.; Azeredo, Joana; Henriques, MarianaAccording to epidemiological data, Candida tropicalis has been related to urinary tract infections and haematological malignancy. Several virulence factors seem to be responsible for C. tropicalis infections, for example: their ability to adhere and to form biofilms onto different indwelling medical devices; their capacity to adhere, invade and damage host human tissues due to enzymes production such as protein...
HC-0C-03: Biological treatments to improve the quality of heavy crude oils
Gudiña, Eduardo J.; Teixeira, J. A.Petroleum or crude oil is a crucial source of energy and one of the main factors driving the Worlds economy. The progressive depletion of high-quality crudes in the last decades will make necessary the exploitation of unconventional low-quality heavy and extra-heavy crudes to meet future energy demands. However, their exploitation requires the application of special techniques in order to facilitate their recov...
Spherical and needle shaped magnetic nanoparticles for friction and magnetic st...
Mendes, Gabriel Pinto; Kluskens, Leon D.; Mota, M.; Lanceros-Méndez, S.; Hatton, T. AlanSupplementary material related to this article can be found online at https://doi.org/10.1016/j.nanoso.2021.100732.; Spherical and needle shaped magnetic nanoparticles (MNPs) were synthesized by thermal decomposition, functionalized with 2-pyrrolidinone for the attachment of pUC19 plasmidic DNA and used in transformations assays of Escherichia coli JM109. Frictional and magnetic stimulation were employed for pr...
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