Project/scholarship details
- Project/scholarship ID
68517
- Reference
PTDC/FIS/68517/2006
- FundRef URI
- Funder
FCT - Fundação para a Ciência e a Tecnologia, I.P.
- Funder's country
Portugal
- Funding program
5876-PPCDTI
- Funding amount
150,824.00 €
- Start date
2007-06-01
- End date
2010-11-30
Documents
Application of fluorescence techniques to the study of protein adsorption and p...
Benesch, J.; Hungerfod, G.; Suhling, K.; Tavares, C. J.; Lewitt, J. A.; Mano, J. F.; Reis, R. L.[Excerpt] The ways proteins compete for the surface of biomaterials and change conformation are believed to be important for the host response to implants. It is possible to elucidate information on packing and any induced conformational change by making use of different fluorescence techniques on fluorescently labelled proteins. Employing probe-probe resonance energy transfer (RET) allows inter and intra prote...
Carrageenan-based hydrogels for the controlled delivery of PDGF-BB in bone tiss...
Santo, Vitor E.; Frias, A. M.; Carida, Michela; Cancedda, Ranieri; Gomes, Manuela E.; Mano, J. F.; Reis, R. L.One of the major drawbacks found in most bone tissue engineering approaches developed so far consists in the lack of strategies to promote vascularisation. Some studies have addressed different issues that may enhance vascularisation in tissue engineered constructs, most of them involving the use of growth factors (GFs) that are involved in the restitution of the vascularity in a damaged zone. The use of sustai...
Immobilization of fibronectin in chitosan substrates improves cell adhesion and...
Custódio, Catarina A.; Alves, C. M.; Reis, R. L.; Mano, J. F.Covalent grafting of biomolecules is a strategy to improve the biocompatibility and bioactivity of materials. However, it is critical to maintain the biological activity of the biomolecule upon its attachment to the surface. In the present study we compared the biological properties of chitosan, in which the surface was enriched with fibronectin (Fn), using two methodologies: chemical immobilization, using a wa...
Controlling cell behavior through the design of polymer surfaces
Alves, N. M.; Pashkuleva, I.; Reis, R. L.; Mano, J. F.Polymers have gained a remarkable place in the biomedical fi eld as materials for the fabrication of various devices and for tissue engineering applications. The initial acceptance or rejection of an implantable device is dictated by the crosstalk of the material surface with the bioentities present in the physiological environment. Advances in microfabrication and nanotechnology offer new tools to investigate ...
Hybrid 3D structure of poly(d,l-lactic acid) loaded with chitosan/chondroitin s...
Santo, Vitor E.; Duarte, Ana Rita C.; Gomes, Manuela E.; Mano, J. F.; Reis, R. L.In the tissue engineering (TE) field, the concept of producing multifunctional scaffolds, capable not only of acting as templates for cell transplantation but also of delivering bioactive agents in a controlled manner, is an emerging strategy aimed to enhance tissue regeneration. In this work, a complex hybrid release system consisting in a three-dimensional (3D) structure based on poly(d,l-lactic acid) (PDLLA)...
Encapsulation of adipose-derived stem cells and transforming growth factor-beta...
Rocha, Pedro M.; Espírito Santo, Vítor; Gomes, Manuela E.; Reis, R. L.; Mano, J. F.Tissue engineering (TE) is an emerging field for the regeneration of damaged tissues. The combination of hydrogels with stem cells and growth factors (GFs) has become a promising approach to promote cartilage regeneration. In this study, carrageenan-based hydrogels were used to encapsulate both cells and transforming growth factor-b1 (TGF-b1). The ATDC5 cell line was encapsulated to determine the cytotoxicity a...
Gellan gum-based hydrogels for intervertebral disc tissue engineering applications
Silva-Correia, Joana; Oliveira, Joaquim M.; Caridade, S. G.; Oliveira, J. T.; Sousa, R. A.; Mano, J. F.; Reis, R. L.Intervertebral disc (IVD) degeneration is a challenging clinical problem that urgently demands viable nucleus pulposus (NP) implant materials. The best suited biomaterial for NP regeneration has yet to be identified, but it is believed that biodegradable hydrogel-based materials are promising candidates. In this work, we have developed ionic- and photo-crosslinked methacrylated gellan gum (GG–MA) hydrogels to b...
Spider silk-bone sialoprotein fusion proteins for bone tissue engineering
Gomes, Sílvia C.; Leonor, I. B.; Mano, J. F.; Reis, R. L.; Kaplan, DavidThe remarkable mechanical characteristics of the spider silk protein major ampullate spidroin protein suggest this polymer as a promising biomaterial to consider for the fabrication of scaffolds for bone regeneration. Herein, a new functionalized spider silk-bone sialoprotein fusion protein was designed, cloned, expressed, purified and the osteogenic activity studied. Bone sialoprotein (BSP) is a multidomain pr...
Genetically engineered new spider silk chimeras for bone regeneration
Pereira, Sílvia Cristina GomesTese de doutoramento em Engenharia de Tecidos, Medicina Regenerativa e Células Estaminais; Tissue engineering and regenerative medicine are emerging fields focused on the development of tissues and organs that can be used in transplantation surgeries for the replacement or repair of damaged tissues or organs. This accomplishment would discard the need to use autologous and allogenic grafts, overcoming problems ...
Development of an injectable system based on elastin-like recombinamer particle...
Oliveira, Mariana B.; Song, Wenlong; Martín, Laura; Oliveira, Sara M.; Caridade, S. G.; Alonso, Matilde; Rodríguez-Cabello, José Carlos; Mano, J. F.An elastin-like recombinamer (ELR) containing the RGD cell adhesion domain was used to fabricate microparticles by an innovative and affordable process based on the use of superhydrophobic surfaces. Two microparticles types with different crosslinking extents were prepared. The biological response was tested using an osteoblast-like cell line (SaOs-2) performing proliferation and alkaline phosphatase (ALP) quan...
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