Modulation of Bax by Protein Kinase C in yeast

New observations on the integrity, structure and physiology of flesh cells from...

The physiological/structural status of the soft ripened berry is still a matter of debate. In this paper isolated mesocarp cells from ripened berries of both wine and table varieties were studied by bright-field, fluorescence and confocal microscopy and flow cytometry to highlight the organization of berry flesh cell, function and viability. Flow cytometry analysis confirmed that protoplasting from grape berry ...

Study of the role of ceramide in acetate-induced cell death in colorectal carci...

Dissertação de mestrado em Genética Molecular; Colorectal carcinoma (CRC) has one of the highest incidences and mortality on the modern society, but a growing impact on less developed and economically transitioning nations. Prevention, surgery and radio/chemotherapy are the principal approaches to overcome the disease. Finding new efficient/specific treatments in CRC, better tolerated by patients is mandatory. ...

Acetate-induced apoptosis in colorectal carcinoma cells involves lysosomal memb...

Colorectal carcinoma (CRC) is one of the most common causes of cancer-related mortality. Short-chain fatty acids secreted by dietary propionibacteria from the intestine, such as acetate, induce apoptosis in CRC cells and may therefore be relevant in CRC prevention and therapy. We previously reported that acetic acid-induced apoptosis in Saccharomyces cerevisiae cells involves partial vacuole permeabilization an...

C2-phytoceramide perturbs lipid rafts and cell integrity in Saccharomyces cerev...

Specific ceramides are key regulators of cell fate, and extensive studies aimed to develop therapies based on ceramide-induced cell death. However, the mechanisms regulating ceramide cytotoxicity are not yet fully elucidated. Since ceramides also regulate growth and stress responses in yeast, we studied how different exogenous ceramides affect yeast cells. C2-phytoceramide, a soluble form of phytoceramides, the...

Colorectal cancer-related mutant KRAS alleles function as positive regulators o...

The recent interest to modulate autophagy in cancer therapy has been hampered by the dual roles of this conserved catabolic process in cancer, highlighting the need for tailored approaches. Since RAS isoforms have been implicated in autophagy regulation and mutation of the KRAS oncogene is highly frequent in colorectal cancer (CRC), we questioned whether/how mutant KRAS alleles regulate autophagy in CRC and its...