Project/scholarship details
- Project/scholarship ID
69795
- Reference
PTDC/QUI/69795/2006
- FundRef URI
- Funder
FCT - Fundação para a Ciência e a Tecnologia, I.P.
- Funder's country
Portugal
- Funding program
5876-PPCDTI
- Funding amount
147,329.00 €
- Start date
2009-01-01
- End date
2012-06-30
Documents
Characterization of mixed DODAB/monoolein aggregates using Nile Red as a solvat...
Silva, J. P. Neves; Oliveira, M. Elisabete; Coutinho, Paulo J. G.The aim of this study was to get a further knowledge of the interactions between lamellar and non-lamellar forming-phospholipids, as a tool to better understand the membrane dynamics and function. We have studied the behaviour of aqueous mixtures of a cationic lipid (dioctadecyldimethylammonium bromide) and a neutral phospholipid (1-monoolein) in 10 to 150 micro molar total lipid concentration range. The effect...
DODAB/Monoolein mixed cationic liposomes for gene delivery : investigation by D...
Oliveira, I. M. S. C.; Silva, J. P. Neves; Feitosa, Eloi; Castanheira, Elisabete M. S.; Oliveira, M. ElisabeteFCT and FEDER for financial support to CFUM and also by funding through project PTDC/QUI/69795/2006 and PhD grant of J.P.N. Silva (SFRH/BD/46968/2009).
Monoolein as helper lipid for non-viral transfection in mammals
Oliveira, Ana Cristina Norberto Gonçalves; Silva, J. P. Neves; Coutinho, Paulo J. G.; Gomes, A. A.; Coutinho, O. P.; Oliveira, M. ElisabeteLipoplexes composed of pDNA and DODAB/MO at different molar ratios (4:1, 2:1 and 1:1) and different cationic lipid/DNA charge ratios were investigated. The physicochemical properties of the lipoplexes (size and charge), the pDNA complexation, and the effect of heparin on pDNA release, were studied by Dynamic Light Scattering, Zeta Potential, and Ethidium Bromide exclusion assays. The cytotoxicity, transfection ...
Monoolein-based lipoplexes (DODAB/MO/DNA) as non-viral vector for transfection-...
Oliveira, Ana Cristina Norberto Gonçalves; Silva, J. P. Neves; Coutinho, Paulo J. G.; Gomes, A. A.; Coutinho, O. P.; Oliveira, M. ElisabeteCationic liposomes/DNA (lipoplexes) have been widely used as non-viral vectors for transfection, the role of the neutral lipid in liposome formulation being determinant for the efficiency of this process [1,2]. In this work, we studied the potential of monoolein (MO) as helper lipid for cellular transfection. Lipoplexes composed of pDNA and dioctadecyldimethylammonium bromide (DODAB)/1-monooleoyl-rac-glycerol (...
DODAB: monoolein-based lipoplexes as non-viral vectors for transfection of mamm...
Silva, J. P. Neves; Oliveira, Ana Cristina Norberto Gonçalves; Casal, Margarida; Gomes, Andreia C; Coutinho, Paulo J. G.; Coutinho, O. P.DNA/Cationic liposome complexes (lipoplexes) have been widely used as non-viral vectors for transfection. Neutral lipids in liposomal formulation are determinant for transfection efficiency using these vectors. In this work, we studied the potential of monoolein (MO) as helper lipid for cellular transfection. Lipoplexes composed of pDNA and dioctadecyldimethylammonium bromide (DODAB)/1-monooleoyl-rac-glycerol (...
Aggregation behavior of aqueous dioctadecyldimethylammonium bromide/monoolein m...
Oliveira, I. M. S. C.; Silva, João P. N.; Feitosa, Eloi; Marques, E. F.; Castanheira, Elisabete M. S.; Oliveira, M. ElisabeteA recently described non-viral gene delivery system [dioctadecyldimethylammonium bromide (DODAB)/ monoolein (MO)] has been studied in detail to improve knowledge on the interactions between lamellar (DODAB) and non-lamellar-forming (MO) lipids, as a means to enhance their final cell transfection efficiency. Indeed, the morphology, fluidity, and size of these cationic surfactant/neutral lipid mixtures play an im...
Development of dioctadecyldimethylammonium bromide/monoolein liposomes for gene...
Silva, João P. N.; Oliveira, Ana Cristina Norberto Gonçalves; Gomes, A. C.; Oliveira, M. ElisabeteThe artificial introduction of nucleic acids (NA) into mammalian cells (transfection) has become, in recent years, a well-established procedure in basic and applied research, which allowed the study of gene function and regulation. The advances in this area have made possible the use of these methods for gene-based medicines, which constitute alternative therapeutic approaches. One of the most prominent methods...
DODAB:monoolein liposomes containing Candida albicans cell wall surface protein...
Carneiro, Catarina; Correia, Alexandra; Collins, Tony; Vilanova, Manuel; Pais, Célia; Gomes, Andreia C; Real Oliveira, M. Elisabete C. D.We describe the preparation and characterization of DODAB:MO-based liposomes and demonstrate their adjuvant potential and use in antigen delivery. Liposomes loaded with Candida albicans proteins assembled as stable negatively charged spherical nanoparticles with a mean size of 280 nm. High adsorption efficiency (91.0 ± 9.0%) is attained with high lipid concentrations. The nanoparticles were non-toxic, avidly ta...
Stealth monoolein-based nanocarriers for delivery of siRNA to cancer cells
Oliveira, Ana Cristina Norberto Gonçalves; Raemdonck, Koen; Martens, Thomas; Rombouts, Koen; Simón-Vázquez, Rosana; Botelho, C. M.While the delivery of small interfering RNAs (siRNAs) is an attractive strategy to treat several clinical con- ditions, siRNA-nanocarriers stability after intravenous administration is still a major obstacle for the development of RNA-interference based therapies. But, although the need for stability is well recognized, the notion that strong stabilization can decrease nanocarriers efficiency is sometimes negle...
Counter ions and constituents combination affect DODAX: MO nanocarriers toxicit...
Oliveira, Ana Cristina Norberto Gonçalves; Sarria, Marisa Passos; Moreira, Pedro; Fernandes, Joana; Castro, Lisandra Marisa Flores; Lopes, IvoLiposomes have received extensive attention as nanocarriers for bioactive compounds due to their good biocompatibility, possibility of targeting and incorporation of hydrophilic and hydrophobic compounds. Although generally considered as safe, detailed knowledge of the effects induced in cells and tissues with which they interact is still underexplored. The aim of this study is to gain insight into the toxicity...
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