Project/scholarship details
- Project/scholarship ID
42688
- Reference
POCTI/ESP/42688/2001
- FundRef URI
- Funder
FCT - Fundação para a Ciência e a Tecnologia, I.P.
- Funder's country
Portugal
- Funding program
POCI
- Funding amount
26,000.00 €
- Start date
2002-03-01
- End date
2005-02-28
Documents
Influence of batch or fed-batch growth on Staphylococcus epidermidis biofilm fo...
Cerca, Nuno; Pier, Gerald B.; Vilanova, Manuel; Oliveira, Rosário; Azeredo, JoanaAims: To make a quantitative evaluation of the differences in biofilm formation by Staphylococcus epidermidis using batch and fed-batch growth systems and to correlate this with production of the major biofilm polysaccharide, poly-N-acetyl glucosamine (PNAG). Methods and Results: Dry weight measurements of biofilms formed in batch and fed-batch conditions were compared with haemagglutination titres, which measu...
Comparative evaluation of coagulase-negative staphylococci (CoNS) adherence to ...
Cerca, Nuno; Pier, Gerald B.; Oliveira, Rosário; Azeredo, JoanaThe main goal of this work was to evaluate the adhesion to acrylic of several clinical strains of Staphylococcus epidermidis and Staphylococcus haemolyticus using both static and dynamic adhesion methods, and to compare the results obtained with these two methods. Adhesion was evaluated using the static slide method with different washing procedures, and the parallel plate flow chamber method. The extent of S. ...
Comparative assessment of antibiotic susceptibility of coagulase-negative staph...
Cerca, Nuno; Martins, Silvia; Cerca, Filipe; Jefferson, Kimberly K.; Pier, Gerald B.; Oliveira, Rosário; Azeredo, JoanaPublished by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy; Objectives: To quantitatively compare the antibiotic susceptibility of biofilms formed by the coagulasenegative staphylococci (CoNS) Staphylococcus epidermidis and Staphylococcus haemolyticus with the susceptibility of planktonic cultures. Methods: SeveralCoNSstrains were grown planktonically or as biofilms to ...
Effects of Growth in the Presence of Subinhibitory Concentrations of Dicloxacil...
Cerca, Nuno; Martins, Silvia; Sillankorva, Sanna; Jefferson, Kimberly K.; Pier, Gerald B.; Oliveira, Rosário; Azeredo, JoanaLow concentrations of antibiotics can inhibit microbial adherence to medical device surfaces. However, little is known about the changes that occur in the physiology of bacteria within biofilms formed in the presence of subinhibitory (sub-MIC) concentrations of antibiotics. In this study, the densities and matrix compositions of biofilms formed by two coagulase-negative Staphylococcus species in the absence and...
Quantitative analysis of adhesion and biofilm formation on hydrophilic and hydr...
Cerca, Nuno; Pier, Gerald B.; Vilanova, Manuel; Oliveira, Rosário; Azeredo, JoanaStaphylococcus epidermidis is now well established as a major nosocomial pathogen associated with infections of indwelling medical devices. The major virulence factor of these organisms is their ability to adhere to devices and form biofilms. However, it has not been established that adherence and biofilm formation are closely linked phenotypes for clinical isolates. In this study, the initial adhesion to diffe...
The relationship between inhibition of bacterial adhesion to a solid surface by...
Cerca, Nuno; Martins, Silvia; Pier, Gerald B.; Oliveira, Rosário; Azeredo, JoanaMany studies have demonstrated that subminimal inhibitory concentrations (sub-MICs) of antibiotics can inhibit initial microbial adherence to medical device surfaces. It has been suggested that, by inhibiting initial adhesion, biofilm formation might be prevented. However, since initial adherence and subsequent biofilm formation may be two distinct phenomena, conclusions regarding the effects of sub-MIC antibio...
Comparative antibody-mediated phagocytosis of Staphylococcus epidermidis cells ...
Cerca, Nuno; Jefferson, Kimberly K.; Oliveira, Rosário; Pier, Gerald B.; Azeredo, JoanaStaphylococcus epidermidis is an important cause of nosocomial infections. Virulence is attributable to elaboration of biofilms on medical surfaces that protect the organisms from immune system clearance. Even though leukocytes can penetrate biofilms, they fail to phagocytose and kill bacteria. The properties that make biofilm bacteria resistant to the immune system are not well characterized. In order to bette...
Staphylococcus epidermidis glucose uptake in biofilm versus planktonic cells
Sousa, Cláudia; Henriques, Mariana; Azeredo, Joana; Teixeira, P.; Oliveira, RosárioThe aim of this work was to compare the glucose uptake of biofilms formed by four different Staphylococcus epidermidis strains as well as to compare between sessile and planktonic cells of the same strain. Biofilm cells showed a lower level of glucose uptake compared to planktonic cells. Moreover, glucose uptake by cells in the sessile form was strongly influenced by biofilm composition. Therefore, this work he...
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