Project/scholarship details
- Project/scholarship ID
56102
- Reference
POCI/AGR/56102/2004
- FundRef URI
- Funder
FCT - Fundação para a Ciência e a Tecnologia, I.P.
- Funder's country
Portugal
- Funding program
POCI
- Funding amount
81,970.00 €
- Start date
2005-07-01
- End date
2008-12-31
Documents
Genetic instability of commercial Saccharomyces cerevisiae strains recovered fr...
Schuller, Dorit Elisabeth; Alves, Hugo; Cambon, Brigitte; Dequin, Sylvie; Casal, MargaridaFrom spontaneous fermentations carried out with grapes collected in vineyards from the Vinho Verde Region, 101 isolates were recovered whose mitochondrial DNA restriction length polymorphism (mtDNA RFLP) were identical to strain Zymaflore VL1, a commercial strain (Lallemand) that has been used by the wineries in the last years. Genetic polymorphisms were evaluated by microsatellite analysis, interdelta sequence...
Isolation and characterization of yeast strains with ability to decrease volati...
Moura, A. Vilela; Schuller, Dorit Elisabeth; Faia, A. Mendes; Côrte-Real, ManuelaThe level of acetic acid, the main component of volatile acidity, is critical for wine quality. Its concentration in wines is approximately 0.5 g/L and, legally, must remain below 1.0g/L. Winemakers use a procedure called “remostagem” to lower acetic acid of wines with high volatile acidity (above 0.8g/L). The spoiled wine is mixed with freshly crushed grapes (1) in a proportion of no more than 20-30% (v/v). Th...
Identification of wine related yeast species by capillary electrophoresis singl...
Dellinger, B.; Graça, Magda Maria Couto da Silva; Casal, Margarida; Schuller, Dorit ElisabethFinancially supported by the programs POCI 2010 (FEDER/FCT, POCTI/AGR/56102/2004, POCTI/AGR/56771/2004), and AGRO (ENOSAFE, Nº 762).
Biochemical and molecular characterization of Saccharomyces cerevisiae strains ...
Oliveira, Valdinéia Aparecida; Vicente, Maristela Araújo; Fietto, L. G.; Castro, I. M.; Coutrim, Maurício Xavier; Schuller, Dorit ElisabethSaccharomyces cerevisiae strains from different regions of Minas Gerais, Brazil, were isolated and characterized aiming at the selection of starter yeasts to be used in the production of cachac¸a, the Brazilian sugar cane spirit. The methodology established took into account the screening for biochemical traits desirable in a yeast cachaça producer, such as no H2S production, high tolerance to ethanol and high ...
Comparative genomics of yeast strains isolated from diverse ecological niches u...
Eiriz, Maria Francisca; Carreto, Laura; Gomes, Ana Catarina; Pereira, Patrícia; Schuller, Dorit Elisabeth; Santos, Manuel A. S.Background: Genome variability generates phenotypic heterogeneity and is of relevance for adaptation to environmental change, but the extent of such variability in natural populations is still poorly understood. For example, selected Saccharomyces cerevisiae strains are variable at the ploidy level, have gene amplifications, changes in chromosome copy number, and gross chromosomal rearrangements. This suggests ...
Reduction of volatile acidity of wines by selected yeast strains
Moura, A. Vilela; Schuller, Dorit Elisabeth; Faia, A. Mendes; Côrte-Real, ManuelaHerein we isolate and characterize wine yeasts with ability to reduce volatile acidity of wines using a refermentation process, which consists in mixing the acidic wine with freshly crushed grapes or musts or, alternatively, in the incubation with the residual marc. From a set of 135 yeast isolates, four strains revealed ability to use glucose and acetic acid simultaneously. Three of them were identified as Sac...
Computational approaches for the genetic and phenotypic characterization of a S...
Duarte, Ricardo Franco; Umek, Lan; Zupan, Blaz; Schuller, Dorit ElisabethWithin this study, we have used a set of computational techniques to relate the genotypes and phenotypes of natural populations of Saccharomyces cerevisiae, using allelic information from 11 microsatellite loci and results from 24 phenotypic tests. A group of 103 strains was obtained from a larger S. cerevisiae winemaking strain collection by clustering with self-organizing maps. These strains were further char...
Effects of acetic acid, ethanol and SO2 on the removal of volatile acidity from...
Moura, A. Vilela; Schuller, Dorit Elisabeth; Faia, A. Mendes; Côrte-Real, ManuelaHerein we report the influence of different combinations of initial concentration of acetic acid and ethanol on the removal of acetic acid from acidic wines by two commercial Saccharomyces cerevisiae strains S26 and S29. Both strains reduced the volatile acidity of an acidic wine (1.0 g l-1 acetic acid and 11% (v/v) ethanol) by 78% and 48%, respectively. Acetic acid removal by both strains was associated with a...
Biogeographical survey of indigenous yeasts from the vineyards of the Azores Ar...
Neves, J. Drumonde; Lima, Maria Teresa; Schuller, Dorit ElisabethThis study aims at the characterization of the fermentative yeasts flora of the vineyards of the Azores islands, for biodiversity preservation and the constitution of a Saccharomyces cerevisiae strain collection as a genetic resource for future strain selection and improvement. During the harvest of 2009, 88 grape samples were collected from eight islands of the archipelago that belonged to traditional (Arinto,...
A colecção de estirpes autóctones de Saccharomyces cerevisiae das principais re...
Vieira, E.; Neves, J. Drumonde; Machado, Raul; Silva, Paulo; Gomes, Ana Catarina; Sousa, Susana; Ramos, P.; Alemão, F.; Lima, Maria TeresaLivro de Actas on-line em http://www.ateva.pt/8-simposio-de-vitivinicultura-do-alentejo/; A levedura Saccharomyces cerevisiae é caracterizada por uma elevada variabilidade fenotípica, que está associada à ocorrência de estirpes em habitats naturais diversificados. Em trabalhos anteriores, demonstrámos a elevada diversidade de estirpes isoladas a partir de ambientes vitivinícolas na Região dos Vinhos Verdes, bem...
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