Project/scholarship details


Mycotoxins are secondary metabolites produced by molds, which are toxic to animals in small amounts and ubiquitous in many agricultural commodities. Since they have been recognized as a potential threat to human and animal health, many countries have regulations to control their presence in food and feed. The occurrence of mycotoxins in fields is inevitable under certain environmental conditions. Therefore, it is recommended that their presence should be reduced as much as technologically possible at the post-harvest level. The present methods and technologies are not always effective on that, and some may even significantly change the nutritional quality of food and feed. Therefore, researchers are still looking for better solutions. Presently, the biological approaches hold a leading role among sought strategies. Their main focus has been to find microorganisms and/or enzymes that can transform or biodegrade mycotoxins into non-toxic compounds. Lactic acid bacteria (LAB) are considered health beneficial microorganisms because they are traditionally used in the production of fermented food and feed products, and because they have several well known probiotic properties. Nevertheless, more recently they also began to be known for their ability to detoxify mycotoxins. This unusual LAB characteristic is strain specific and involves adsorption and metabolization mechanisms. Presently, active strains are very rare and the involved mechanisms are not yet fully understood and clarified by researchers. They may be used as probiotic for animal nutrition, as inoculants for silages, as biocontrol agents in crop fields, as starter cultures for fermented products, and/or as starter cultures for wine malolactic fermentations. The present project intends to explore the potential of LAB to detoxify mycotoxins in food products and to elucidate some fundamental research questions on this subject.

  • Funder

    FCT - Fundação para a Ciência e a Tecnologia, I.P.

  • Funder's country

    Portugal

  • Funding program

    5876-PPCDTI

  • Funding amount

    96,054.00 €

  • Start date

    2013-05-01

  • End date

    2015-07-31

Documents


Analytical evaluation of fining treatments for white wines contaminated by Ochr...

Carvalho, Filipa; Inês, António; Nunes, Fernando M.; Filipe-Ribeiro, Luís; Abrunhosa, Luís; Cosme, Fernanda

Mycotoxins are toxic secondary metabolites produced by certain molds. Ochratoxin A (OTA) is one of the most relevant. Its chemical structure is a dihydro-isocoumarin connected at the 7-carboxy group to a molecule of L--phenylalanine via an amide bond. OTA in wine is a risk to consumer health [1]. According to the Regulation No. 123/2005 of the European Commission, the maximum limit for OTA in wine is 2 µg/kg [2...


Use of Pediococcus parvulus to remove ochratoxin A from wine

Guimarães, Ana Cristina Silva Esperança; Pereira, Vânia L.; Inês, António; Abrunhosa, Luís

A ocratoxina A (OTA) é uma micotoxina que pode ser encontrada no café, vinho tinto e frutos secos mas a sua principal fonte são os cereais e os seus derivados. É produzida principalmente por Penicillium verrucosum, Aspergillus westerdijkiae, Aspergillus carbonarius e Aspergillus niger. Estas duas últimas espécies são muito comuns em uvas e são responsáveis pela presença de OTA nos vinhos. Na Europa, o vinho é a...


Biodegradation of ochratoxin A by Pediococcus parvulus isolated from Douro wines

Abrunhosa, Luís; Inês, António; Rodrigues, A. I.; Guimarães, Ana Cristina Silva Esperança; Pereira, Vânia L.; Parpot, Pier; Mendes-Faia, Arlete

Lactic acid bacteria (LAB) are a promising solution to reduce exposure to dietary mycotoxins because of the unique mycotoxin decontaminating characteristic of some LAB. Ochratoxin A (OTA) is one of the most prominent mycotoxins found in agricultural commodities. The present work reports on the ability of Pediococcus parvulus strains that were isolated from Douro wines that spontaneously underwent malolactic fer...


Biodegradação de Ochratoxin A por Pediococcus parvulus

Abrunhosa, Luís; Guimarães, Ana Cristina Silva Esperança; Inês, António; Venâncio, Armando

Algumas bactérias do ácido láctico (BAL) são capazes de destoxificar micotoxinas através de processos de adsorção às suas paredes celulares ou através de processos de biotransformação em compostos menos tóxicos. Uma das micotoxinas mais importantes encontradas em produtos agrícolas é a ocratoxina A (OTA). A OTA é conhecida principalmente pela sua nefro e carcinogenicidade, estando classificada no Grupo 2B pelo ...


Avaliação da eficiência de diferentes produtos enológicos na remoção de ocratox...

Carvalho F.; Inês, A.; Nunes, F.; Filipe-Ribeiro, L.; Abrunhosa, Luís; Cosme, F.

As micotoxinas são metabolitos secundários tóxicos produzidos por certos fungos, sendo a ocratoxina A (OTA) das mais importantes. A presença de OTA nos vinhos pode constituir um risco para a saúde dos consumidores, sendo por isso aconselhado que se tomem medidas para atingir níveis seguros para o consumo humano [1]. De acordo com o Regulamento n.º 1881/2006 da Comissão Europeia, o limite máximo para a OTA em vi...


Ochratoxin A biodegradation by pediococcus parvulus

Abrunhosa, Luís; Guimarães, Ana Cristina Silva Esperança; Inês, António; Venâncio, Armando

Some lactic acid bacteria (LAB) have mycotoxin decontaminating properties due to the absorbing characteristics of their cells walls and because some can biotransform mycotoxins into less toxic compounds. One importante mycotoxin found in agricultural commodities is ochratoxin A (OTA). OTA is known mainly for its nephrotoxicity and carcinogenicity being classified in Group 2B by IARC. The present work reports on...


Avaliação da eficiência de diferentes carvões ativados comerciais na remoção de...

Silva, Davide; Inês, António; Nunes, Fernando M.; Filipe-Ribeiro, Luís; Abrunhosa, Luís; Cosme, Fernanda

A presença de micotoxinas em alimentos é uma preocupação ao nível da segurança alimentar. Contudo, os vinhos não são exceção podendo também estar contaminados com micotoxinas, especialmente ocratoxina A (OTA) [1]. Como estas substâncias tóxicas não podem ser completamente removidas da cadeia alimentar, muitos países definiram limites legais nos alimentos, com o intuito de aumentar a segurança alimentar. Assim, ...


Zearalenone and its derivatives α-Zearalenol and β-Zearalenol decontamination b...

Keller, Luiz; Abrunhosa, Luís; Keller, Kelly; Rosa, Carlos Alberto; Cavaglieri, Lilia; Venâncio, Armando

Zearalenone (ZEA) and its derivatives are mycotoxins with estrogenic effects on mammals. The biotransformation for ZEA in animals involves the formation of two major metabolites, - and -zearalenol (-ZOL and -ZOL), which are subsequently conjugated with glucuronic acid. The capability of Saccharomyces cerevisiae strains isolated from silage to eliminate ZEA and its derivatives -ZOL and -ZOL was investigated as, ...


Influence of different activated carbons on Ochratoxin A decrease in wines

Inês, António; Silva, Davide; Nunes, Fernando M.; Filipe-Ribeiro, Luís; Abrunhosa, Luís; Cosme, Fernanda

The presence of mycotoxins in foodstuff is a matter of concern for food safety. Wines can also be contaminated with these toxicants. Several authors have demonstrated the presence of mycotoxins in wine, especially ochratoxin A (OTA) [1]. As these toxicants can never be completely removed from the food chain, many countries have defined levels in food in order to attend health concerns. The maximum acceptable le...


Micotoxinas no vinho: problemas e soluções

Abrunhosa, Luís

Financiado pelo FEDER através do COMPETE e pela FCT (Ref. FCOMP-01-0124-FEDER-028029 e PTDC/AGR-TEC/3900/2012, respetivamente). Incentivo/EQB/LA0023/2014 do programa ON.2 – O Novo Norte.

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